Designed surface topographies control ICAM-1 expression in tonsil-derived human stromal cells

Aliaksei S. Vasilevich, Frederic Mourcin, Anouk Mentink, Frits Hulshof, Nick Beijer, Yiping Zhao, Marloes Levers, Bernke Papenburg, Shantanu Singh, Anne E. Carpenter, Dimitrios Stamatialis, Clemens van Blitterswijk, Karin Tarte, Jan de Boer (Corresponding author)

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Fibroblastic reticular cells (FRCs), the T-cell zone stromal cell subtype in the lymph nodes, create a scaffold for adhesion and migration of immune cells, thus allowing them to communicate. Although known to be important for the initiation of immune responses, studies about FRCs and their interactions have been impeded because FRCs are limited in availability and lose their function upon culture expansion. To circumvent these limitations, stromal cell precursors can be mechanotranduced to form mature FRCs. Here, we used a library of designed surface topographies to trigger FRC differentiation from tonsil-derived stromal cells (TSCs). Undifferentiated TSCs were seeded on a TopoChip containing 2176 different topographies in culture medium without differentiation factors, then monitored cell morphology and the levels of ICAM-1, a marker of FRC differentiation. We identified 112 and 72 surfaces that upregulated and downregulated, respectively, ICAM-1 expression. By monitoring cell morphology, and expression of the FRC differentiation marker ICAM-1 via image analysis and machine learning, we discovered correlations between ICAM-1 expression, cell shape and design of surface topographies and confirmed our findings by using flow cytometry. Our findings confirmed that TSCs are mechano-responsive cells and identified particular topographies that can be used to improve FRC differentiation protocols.
TaalEngels
Artikelnummer87
Aantal pagina's14
TijdschriftFrontiers in Bioengineering and Biotechnology
Volume6
DOI's
StatusGepubliceerd - 28 jun 2018

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    Vasilevich, Aliaksei S. ; Mourcin, Frederic ; Mentink, Anouk ; Hulshof, Frits ; Beijer, Nick ; Zhao, Yiping ; Levers, Marloes ; Papenburg, Bernke ; Singh, Shantanu ; Carpenter, Anne E. ; Stamatialis, Dimitrios ; van Blitterswijk, Clemens ; Tarte, Karin ; de Boer, Jan. / Designed surface topographies control ICAM-1 expression in tonsil-derived human stromal cells. In: Frontiers in Bioengineering and Biotechnology. 2018 ; Vol. 6.
    @article{1eb07f8d31da42cf9b1cf664c5a3bd1a,
    title = "Designed surface topographies control ICAM-1 expression in tonsil-derived human stromal cells",
    abstract = "Fibroblastic reticular cells (FRCs), the T-cell zone stromal cell subtype in the lymph nodes, create a scaffold for adhesion and migration of immune cells, thus allowing them to communicate. Although known to be important for the initiation of immune responses, studies about FRCs and their interactions have been impeded because FRCs are limited in availability and lose their function upon culture expansion. To circumvent these limitations, stromal cell precursors can be mechanotranduced to form mature FRCs. Here, we used a library of designed surface topographies to trigger FRC differentiation from tonsil-derived stromal cells (TSCs). Undifferentiated TSCs were seeded on a TopoChip containing 2176 different topographies in culture medium without differentiation factors, then monitored cell morphology and the levels of ICAM-1, a marker of FRC differentiation. We identified 112 and 72 surfaces that upregulated and downregulated, respectively, ICAM-1 expression. By monitoring cell morphology, and expression of the FRC differentiation marker ICAM-1 via image analysis and machine learning, we discovered correlations between ICAM-1 expression, cell shape and design of surface topographies and confirmed our findings by using flow cytometry. Our findings confirmed that TSCs are mechano-responsive cells and identified particular topographies that can be used to improve FRC differentiation protocols.",
    keywords = "mechanobiology, surface topography, fibroblastic reticular cells, lymph node, ICAM-1",
    author = "Vasilevich, {Aliaksei S.} and Frederic Mourcin and Anouk Mentink and Frits Hulshof and Nick Beijer and Yiping Zhao and Marloes Levers and Bernke Papenburg and Shantanu Singh and Carpenter, {Anne E.} and Dimitrios Stamatialis and {van Blitterswijk}, Clemens and Karin Tarte and {de Boer}, Jan",
    year = "2018",
    month = "6",
    day = "28",
    doi = "10.3389/fbioe.2018.00087",
    language = "English",
    volume = "6",
    journal = "Frontiers in Bioengineering and Biotechnology",
    issn = "2296-4185",
    publisher = "Frontiers Research Foundation",

    }

    Vasilevich, AS, Mourcin, F, Mentink, A, Hulshof, F, Beijer, N, Zhao, Y, Levers, M, Papenburg, B, Singh, S, Carpenter, AE, Stamatialis, D, van Blitterswijk, C, Tarte, K & de Boer, J 2018, 'Designed surface topographies control ICAM-1 expression in tonsil-derived human stromal cells' Frontiers in Bioengineering and Biotechnology, vol. 6, 87. DOI: 10.3389/fbioe.2018.00087

    Designed surface topographies control ICAM-1 expression in tonsil-derived human stromal cells. / Vasilevich, Aliaksei S.; Mourcin, Frederic; Mentink, Anouk; Hulshof, Frits; Beijer, Nick; Zhao, Yiping; Levers, Marloes; Papenburg, Bernke; Singh, Shantanu; Carpenter, Anne E.; Stamatialis, Dimitrios; van Blitterswijk, Clemens; Tarte, Karin; de Boer, Jan (Corresponding author).

    In: Frontiers in Bioengineering and Biotechnology, Vol. 6, 87, 28.06.2018.

    Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

    TY - JOUR

    T1 - Designed surface topographies control ICAM-1 expression in tonsil-derived human stromal cells

    AU - Vasilevich,Aliaksei S.

    AU - Mourcin,Frederic

    AU - Mentink,Anouk

    AU - Hulshof,Frits

    AU - Beijer,Nick

    AU - Zhao,Yiping

    AU - Levers,Marloes

    AU - Papenburg,Bernke

    AU - Singh,Shantanu

    AU - Carpenter,Anne E.

    AU - Stamatialis,Dimitrios

    AU - van Blitterswijk,Clemens

    AU - Tarte,Karin

    AU - de Boer,Jan

    PY - 2018/6/28

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    N2 - Fibroblastic reticular cells (FRCs), the T-cell zone stromal cell subtype in the lymph nodes, create a scaffold for adhesion and migration of immune cells, thus allowing them to communicate. Although known to be important for the initiation of immune responses, studies about FRCs and their interactions have been impeded because FRCs are limited in availability and lose their function upon culture expansion. To circumvent these limitations, stromal cell precursors can be mechanotranduced to form mature FRCs. Here, we used a library of designed surface topographies to trigger FRC differentiation from tonsil-derived stromal cells (TSCs). Undifferentiated TSCs were seeded on a TopoChip containing 2176 different topographies in culture medium without differentiation factors, then monitored cell morphology and the levels of ICAM-1, a marker of FRC differentiation. We identified 112 and 72 surfaces that upregulated and downregulated, respectively, ICAM-1 expression. By monitoring cell morphology, and expression of the FRC differentiation marker ICAM-1 via image analysis and machine learning, we discovered correlations between ICAM-1 expression, cell shape and design of surface topographies and confirmed our findings by using flow cytometry. Our findings confirmed that TSCs are mechano-responsive cells and identified particular topographies that can be used to improve FRC differentiation protocols.

    AB - Fibroblastic reticular cells (FRCs), the T-cell zone stromal cell subtype in the lymph nodes, create a scaffold for adhesion and migration of immune cells, thus allowing them to communicate. Although known to be important for the initiation of immune responses, studies about FRCs and their interactions have been impeded because FRCs are limited in availability and lose their function upon culture expansion. To circumvent these limitations, stromal cell precursors can be mechanotranduced to form mature FRCs. Here, we used a library of designed surface topographies to trigger FRC differentiation from tonsil-derived stromal cells (TSCs). Undifferentiated TSCs were seeded on a TopoChip containing 2176 different topographies in culture medium without differentiation factors, then monitored cell morphology and the levels of ICAM-1, a marker of FRC differentiation. We identified 112 and 72 surfaces that upregulated and downregulated, respectively, ICAM-1 expression. By monitoring cell morphology, and expression of the FRC differentiation marker ICAM-1 via image analysis and machine learning, we discovered correlations between ICAM-1 expression, cell shape and design of surface topographies and confirmed our findings by using flow cytometry. Our findings confirmed that TSCs are mechano-responsive cells and identified particular topographies that can be used to improve FRC differentiation protocols.

    KW - mechanobiology

    KW - surface topography

    KW - fibroblastic reticular cells

    KW - lymph node

    KW - ICAM-1

    U2 - 10.3389/fbioe.2018.00087

    DO - 10.3389/fbioe.2018.00087

    M3 - Article

    VL - 6

    JO - Frontiers in Bioengineering and Biotechnology

    T2 - Frontiers in Bioengineering and Biotechnology

    JF - Frontiers in Bioengineering and Biotechnology

    SN - 2296-4185

    M1 - 87

    ER -