We propose and experimentally demonstrate a Software-Defined Network (SDN) enabled Hybrid Polling Algorithm for packet Contention Resolution (HPACR) in optical Data Center Network (DCN) based on Fast Optical Switches (FOS). Fast Semiconductor Optical Amplifier (SOA)-based buffer-less FOS with nanoseconds reconfiguration time are employed for interconnecting Top-Of-the Racks (TORs) switches with distributed flow control. Under the realistic 10 Gb/s server traffic, the network performance of the DCN employing the proposed HPACR algorithm is experimentally assessed. Results show that the HPACR increases 13.6% throughput at the high load of 1.0 and decreases the 75% contention ratio for the load >0.5 with respect to the traditional packet contention resolution methods. Based on the experimental parameters, a simulation platform is built to numerically evaluate the HPACR performance of DCN with 64 TORs connecting 1280 servers. The results show that HPACR improves the system performance by increasing the throughput by 5% compared with existing algorithms, reducing the TOR-to-TOR latency by 18.5%, and decreasing the packet loss by 14.1% at the load of 1.