Defining the threshold and boundaries of the keratinocytes response to blue light exposure

Blue light (BL) irradiation of human keratinocytes is known to decrease their proliferation and increase their differentiation without inducing apoptosis in a wavelength and fluence dependent manner. It has been suggested that these BL effects are mediated by nitric oxide (NO) and reactive oxygen species (ROS). However, little is known of the threshold and boundaries of the keratinocytes response to blue light. From in vitro studies at higher wavelengths, it is known that a cell’s redox potential is inversely correlated with the magnitude of the irradiation’s effect. Characterizing the cellular response of these epidermal cells to BL may contribute to the design of effective therapies for inflammatory skin conditions. In order to define the keratinocytes response to blue light exposure and provide a holistic view of the underlying mechanism of BL irradiation we propose a computational model-based method.