TY - JOUR
T1 - Decreased mechanical properties of heart valve tissue constructs cultured in platelet lysate as compared to fetal bovine serum
AU - Geemen, van, D.
AU - Riem Vis, P.W.
AU - Soekhradj - Soechit, R.S.
AU - Sluijter, J.P.G.
AU - Liefde - van Beest, de, M.
AU - Kluin, J.
AU - Bouten, C.V.C.
PY - 2011
Y1 - 2011
N2 - In autologous heart valve tissue engineering, there is an ongoing search for alternatives of fetal bovine serum (FBS). Human platelet-lysate (PL) might be a promising substitute. In the present article, we aimed to examine the tissue formation, functionality, and mechanical properties of engineered three-dimensional tissue constructs cultured in PL as a substitute for FBS. Our results show that tissue constructs that were cultured in PL and FBS produce similar amounts of collagen, glycosoaminoglycans, and collagen crosslinks, and that the cellular phenotype remains unchanged. Nevertheless, mechanical testing showed that the ultimate tensile strength in PL constructs was on average approximately three times lower as compared to FBS (0.25 vs. 0.74¿MPa, respectively, p
AB - In autologous heart valve tissue engineering, there is an ongoing search for alternatives of fetal bovine serum (FBS). Human platelet-lysate (PL) might be a promising substitute. In the present article, we aimed to examine the tissue formation, functionality, and mechanical properties of engineered three-dimensional tissue constructs cultured in PL as a substitute for FBS. Our results show that tissue constructs that were cultured in PL and FBS produce similar amounts of collagen, glycosoaminoglycans, and collagen crosslinks, and that the cellular phenotype remains unchanged. Nevertheless, mechanical testing showed that the ultimate tensile strength in PL constructs was on average approximately three times lower as compared to FBS (0.25 vs. 0.74¿MPa, respectively, p
U2 - 10.1089/ten.tec.2010.0556
DO - 10.1089/ten.tec.2010.0556
M3 - Article
SN - 1937-3384
VL - 17
SP - 607
EP - 617
JO - Tissue Engineering. Part C: Methods
JF - Tissue Engineering. Part C: Methods
IS - 5
ER -