Coordinated collective migration and asymmetric cell division in confluent human keratinocytes without wounding

Emma Lång, Anna Połeć, Anna Lång, Marijke Valk, Pernille Blicher, Alexander D. Rowe, Kim A. Tønseth, Catherine J. Jackson, Tor P. Utheim, Liesbeth M.C. Janssen, Jens Eriksson, Stig Ove Bøe

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Uittreksel

Epithelial sheet spreading is a fundamental cellular process that must be coordinated with cell division and differentiation to restore tissue integrity. Here we use consecutive serum deprivation and re-stimulation to reconstruct biphasic collective migration and proliferation in cultured sheets of human keratinocytes. In this system, a burst of long-range coordinated locomotion is rapidly generated throughout the cell sheet in the absence of wound edges. Migrating cohorts reach correlation lengths of several millimeters and display dependencies on epidermal growth factor receptor-mediated signaling, self-propelled polarized migration, and a G1/G0 cell cycle environment. The migration phase is temporally and spatially aligned with polarized cell divisions characterized by pre-mitotic nuclear migration to the cell front and asymmetric partitioning of nuclear promyelocytic leukemia bodies and lysosomes to opposite daughter cells. This study investigates underlying mechanisms contributing to the stark contrast between cells in a static quiescent state compared to the long-range coordinated collective migration seen in contact with blood serum.

TaalEngels
Artikelnummer3665
Aantal pagina's15
TijdschriftNature Communications
Volume9
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 1 dec 2018

Vingerafdruk

Asymmetric Cell Division
cell division
Keratinocytes
Cells
Cell Division
cells
Locomotion
Lysosomes
Serum
Epidermal Growth Factor Receptor
Cell Movement
Cell Differentiation
deprivation
Cell Cycle
blood serum
Leukemia
lysosomes
Blood
Display devices
leukemias

Citeer dit

Lång, E., Połeć, A., Lång, A., Valk, M., Blicher, P., Rowe, A. D., ... Bøe, S. O. (2018). Coordinated collective migration and asymmetric cell division in confluent human keratinocytes without wounding. Nature Communications, 9(1), [3665]. DOI: 10.1038/s41467-018-05578-7
Lång, Emma ; Połeć, Anna ; Lång, Anna ; Valk, Marijke ; Blicher, Pernille ; Rowe, Alexander D. ; Tønseth, Kim A. ; Jackson, Catherine J. ; Utheim, Tor P. ; Janssen, Liesbeth M.C. ; Eriksson, Jens ; Bøe, Stig Ove. / Coordinated collective migration and asymmetric cell division in confluent human keratinocytes without wounding. In: Nature Communications. 2018 ; Vol. 9, Nr. 1.
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abstract = "Epithelial sheet spreading is a fundamental cellular process that must be coordinated with cell division and differentiation to restore tissue integrity. Here we use consecutive serum deprivation and re-stimulation to reconstruct biphasic collective migration and proliferation in cultured sheets of human keratinocytes. In this system, a burst of long-range coordinated locomotion is rapidly generated throughout the cell sheet in the absence of wound edges. Migrating cohorts reach correlation lengths of several millimeters and display dependencies on epidermal growth factor receptor-mediated signaling, self-propelled polarized migration, and a G1/G0 cell cycle environment. The migration phase is temporally and spatially aligned with polarized cell divisions characterized by pre-mitotic nuclear migration to the cell front and asymmetric partitioning of nuclear promyelocytic leukemia bodies and lysosomes to opposite daughter cells. This study investigates underlying mechanisms contributing to the stark contrast between cells in a static quiescent state compared to the long-range coordinated collective migration seen in contact with blood serum.",
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Lång, E, Połeć, A, Lång, A, Valk, M, Blicher, P, Rowe, AD, Tønseth, KA, Jackson, CJ, Utheim, TP, Janssen, LMC, Eriksson, J & Bøe, SO 2018, 'Coordinated collective migration and asymmetric cell division in confluent human keratinocytes without wounding' Nature Communications, vol. 9, nr. 1, 3665. DOI: 10.1038/s41467-018-05578-7

Coordinated collective migration and asymmetric cell division in confluent human keratinocytes without wounding. / Lång, Emma; Połeć, Anna; Lång, Anna; Valk, Marijke; Blicher, Pernille; Rowe, Alexander D.; Tønseth, Kim A.; Jackson, Catherine J.; Utheim, Tor P.; Janssen, Liesbeth M.C.; Eriksson, Jens; Bøe, Stig Ove.

In: Nature Communications, Vol. 9, Nr. 1, 3665, 01.12.2018.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

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Lång E, Połeć A, Lång A, Valk M, Blicher P, Rowe AD et al. Coordinated collective migration and asymmetric cell division in confluent human keratinocytes without wounding. Nature Communications. 2018 dec 1;9(1). 3665. Beschikbaar vanaf, DOI: 10.1038/s41467-018-05578-7