Control of calcium phosphate nucleation and transformation through interactions of enamelin and amelogenin exhibits the "goldilocks effect"

Jinhui Tao, Andreas Fijneman, Jiaqi Wan, Saumya Prajapati, Kaushik Mukherjee, Alejandro Fernandez-Martinez, Janet Moradian-Oldak, James J. De Yoreo

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Uittreksel

Although amelogenin comprises the vast majority of the matrix that templates calcium phosphate nucleation during enamel formation, other proteins, particularly enamelin, are also known to play an important role in the formation of enamel's intricate architecture. However, there is little understanding of the interplay between amelogenin and enamelin in controlling processes of mineral nucleation and growth. Here, we used an in vitro model to investigate the impact of enamelin interaction with amelogenin on calcium phosphate nucleation for a range of enamelin-to-amelogenin ratios. We found that amelogenin alone is a weak promoter of nucleation, but addition of enamelin enhanced nucleation rates in a highly nonlinear, nonmonotonic manner reaching a sharp maximum at a ratio of 1:50 enamelin/amelogenin. We provide a phenomenological model to explain this effect that assumes only isolated enamelin proteins can act as sites of enhanced nucleation, while enamelin oligomers cannot. Even when interaction is random, the model reproduces the observed behavior, suggesting a simple means to tightly control the timing and extent of nucleation and phase transformation by amelogenin and enamelin.

TaalEngels
Pagina's7391-7400
TijdschriftCrystal Growth and Design
Volume18
Nummer van het tijdschrift2
Vroegere onlinedatum22 okt 2018
DOI's
StatusGepubliceerd - 1 dec 2018

Vingerafdruk

Amelogenin
calcium phosphates
Calcium phosphate
Nucleation
nucleation
interactions
enamels
Enamels
proteins
Proteins
tuftelin
calcium phosphate
oligomers
Oligomers
phase transformations
templates
Minerals
Phase transitions
time measurement
minerals

Citeer dit

Tao, Jinhui ; Fijneman, Andreas ; Wan, Jiaqi ; Prajapati, Saumya ; Mukherjee, Kaushik ; Fernandez-Martinez, Alejandro ; Moradian-Oldak, Janet ; De Yoreo, James J./ Control of calcium phosphate nucleation and transformation through interactions of enamelin and amelogenin exhibits the "goldilocks effect". In: Crystal Growth and Design. 2018 ; Vol. 18, Nr. 2. blz. 7391-7400
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abstract = "Although amelogenin comprises the vast majority of the matrix that templates calcium phosphate nucleation during enamel formation, other proteins, particularly enamelin, are also known to play an important role in the formation of enamel's intricate architecture. However, there is little understanding of the interplay between amelogenin and enamelin in controlling processes of mineral nucleation and growth. Here, we used an in vitro model to investigate the impact of enamelin interaction with amelogenin on calcium phosphate nucleation for a range of enamelin-to-amelogenin ratios. We found that amelogenin alone is a weak promoter of nucleation, but addition of enamelin enhanced nucleation rates in a highly nonlinear, nonmonotonic manner reaching a sharp maximum at a ratio of 1:50 enamelin/amelogenin. We provide a phenomenological model to explain this effect that assumes only isolated enamelin proteins can act as sites of enhanced nucleation, while enamelin oligomers cannot. Even when interaction is random, the model reproduces the observed behavior, suggesting a simple means to tightly control the timing and extent of nucleation and phase transformation by amelogenin and enamelin.",
author = "Jinhui Tao and Andreas Fijneman and Jiaqi Wan and Saumya Prajapati and Kaushik Mukherjee and Alejandro Fernandez-Martinez and Janet Moradian-Oldak and {De Yoreo}, {James J.}",
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Tao, J, Fijneman, A, Wan, J, Prajapati, S, Mukherjee, K, Fernandez-Martinez, A, Moradian-Oldak, J & De Yoreo, JJ 2018, 'Control of calcium phosphate nucleation and transformation through interactions of enamelin and amelogenin exhibits the "goldilocks effect"' Crystal Growth and Design, vol. 18, nr. 2, blz. 7391-7400. DOI: 10.1021/acs.cgd.8b01066

Control of calcium phosphate nucleation and transformation through interactions of enamelin and amelogenin exhibits the "goldilocks effect". / Tao, Jinhui; Fijneman, Andreas; Wan, Jiaqi; Prajapati, Saumya; Mukherjee, Kaushik; Fernandez-Martinez, Alejandro; Moradian-Oldak, Janet; De Yoreo, James J.

In: Crystal Growth and Design, Vol. 18, Nr. 2, 01.12.2018, blz. 7391-7400.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

TY - JOUR

T1 - Control of calcium phosphate nucleation and transformation through interactions of enamelin and amelogenin exhibits the "goldilocks effect"

AU - Tao,Jinhui

AU - Fijneman,Andreas

AU - Wan,Jiaqi

AU - Prajapati,Saumya

AU - Mukherjee,Kaushik

AU - Fernandez-Martinez,Alejandro

AU - Moradian-Oldak,Janet

AU - De Yoreo,James J.

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