Confocal Raman spectroscopy: Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivio

Christian J.F. Bertens (Corresponding author), Shuo Zhang, Roel J. Erckens, Frank J.H.M. van den Biggelaar, Tos T.J.M. Berendschot, Carroll A.B. Webers, Rudy M.M.A. Nuijts, Marlies Gijs

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

Uittreksel

Current information about the pharmacokinetics of an ocular drug can only be achieved by invasive sampling. However, confocal Raman spectroscopy bears the potential to quantify drug concentrations non-invasively. In this project, we evaluated the detection and quantification of ocular ketorolac tromethamine levels with confocal Raman spectroscopy after topical administration.

Confocal Raman spectroscopy and high-performance liquid chromatography (HPLC) were compared in terms of sensitivity of detection. Enucleated pig eyes were treated with different concentrations of ketorolac. Hereafter, ketorolac concentrations in the aqueous humor of pig eyes were analyzed by confocal Raman spectroscopy and HPLC.

Subsequently, twelve rabbits were treated with Acular™ for four weeks. At several time points, ketorolac concentrations in aqueous humor of the rabbits were measured by confocal Raman spectroscopy followed by drawing an aqueous humor sample for HPLC analysis.

In ketorolac treated pig eyes, both ex vivo Raman spectroscopy as well as HPLC were able to detect ketorolac in a broad concentration range. However, in vivo confocal Raman spectroscopy in rabbits was unable to detect ketorolac in contrast to HPLC.

To conclude, confocal Raman spectroscopy has the capacity to detect ketorolac tromethamine in vitro, but currently lacks sensitivity for in vivo detection.
TaalEngels
Artikelnummer118641
Aantal pagina's9
TijdschriftInternational Journal of Pharmaceutics
Volume570
DOI's
StatusGepubliceerd - 30 okt 2019

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    Citeer dit

    Bertens, Christian J.F. ; Zhang, Shuo ; Erckens, Roel J. ; van den Biggelaar, Frank J.H.M. ; Berendschot, Tos T.J.M. ; Webers, Carroll A.B. ; Nuijts, Rudy M.M.A. ; Gijs, Marlies. / Confocal Raman spectroscopy : Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivio. In: International Journal of Pharmaceutics. 2019 ; Vol. 570.
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    title = "Confocal Raman spectroscopy: Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivio",
    abstract = "Current information about the pharmacokinetics of an ocular drug can only be achieved by invasive sampling. However, confocal Raman spectroscopy bears the potential to quantify drug concentrations non-invasively. In this project, we evaluated the detection and quantification of ocular ketorolac tromethamine levels with confocal Raman spectroscopy after topical administration.Confocal Raman spectroscopy and high-performance liquid chromatography (HPLC) were compared in terms of sensitivity of detection. Enucleated pig eyes were treated with different concentrations of ketorolac. Hereafter, ketorolac concentrations in the aqueous humor of pig eyes were analyzed by confocal Raman spectroscopy and HPLC.Subsequently, twelve rabbits were treated with Acular™ for four weeks. At several time points, ketorolac concentrations in aqueous humor of the rabbits were measured by confocal Raman spectroscopy followed by drawing an aqueous humor sample for HPLC analysis.In ketorolac treated pig eyes, both ex vivo Raman spectroscopy as well as HPLC were able to detect ketorolac in a broad concentration range. However, in vivo confocal Raman spectroscopy in rabbits was unable to detect ketorolac in contrast to HPLC.To conclude, confocal Raman spectroscopy has the capacity to detect ketorolac tromethamine in vitro, but currently lacks sensitivity for in vivo detection.",
    keywords = "Raman spectroscopy, High-performance liquid chromatography, In vivo drug detection, Ketorolac tromethamine, Non-invasive drug detection",
    author = "Bertens, {Christian J.F.} and Shuo Zhang and Erckens, {Roel J.} and {van den Biggelaar}, {Frank J.H.M.} and Berendschot, {Tos T.J.M.} and Webers, {Carroll A.B.} and Nuijts, {Rudy M.M.A.} and Marlies Gijs",
    year = "2019",
    month = "10",
    day = "30",
    doi = "10.1016/j.ijpharm.2019.118641",
    language = "English",
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    Confocal Raman spectroscopy : Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivio. / Bertens, Christian J.F. (Corresponding author); Zhang, Shuo; Erckens, Roel J.; van den Biggelaar, Frank J.H.M.; Berendschot, Tos T.J.M.; Webers, Carroll A.B.; Nuijts, Rudy M.M.A.; Gijs, Marlies.

    In: International Journal of Pharmaceutics, Vol. 570, 118641, 30.10.2019.

    Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

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    AU - Erckens,Roel J.

    AU - van den Biggelaar,Frank J.H.M.

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    AU - Webers,Carroll A.B.

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    AB - Current information about the pharmacokinetics of an ocular drug can only be achieved by invasive sampling. However, confocal Raman spectroscopy bears the potential to quantify drug concentrations non-invasively. In this project, we evaluated the detection and quantification of ocular ketorolac tromethamine levels with confocal Raman spectroscopy after topical administration.Confocal Raman spectroscopy and high-performance liquid chromatography (HPLC) were compared in terms of sensitivity of detection. Enucleated pig eyes were treated with different concentrations of ketorolac. Hereafter, ketorolac concentrations in the aqueous humor of pig eyes were analyzed by confocal Raman spectroscopy and HPLC.Subsequently, twelve rabbits were treated with Acular™ for four weeks. At several time points, ketorolac concentrations in aqueous humor of the rabbits were measured by confocal Raman spectroscopy followed by drawing an aqueous humor sample for HPLC analysis.In ketorolac treated pig eyes, both ex vivo Raman spectroscopy as well as HPLC were able to detect ketorolac in a broad concentration range. However, in vivo confocal Raman spectroscopy in rabbits was unable to detect ketorolac in contrast to HPLC.To conclude, confocal Raman spectroscopy has the capacity to detect ketorolac tromethamine in vitro, but currently lacks sensitivity for in vivo detection.

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    KW - High-performance liquid chromatography

    KW - In vivo drug detection

    KW - Ketorolac tromethamine

    KW - Non-invasive drug detection

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