TY - JOUR
T1 - Column liquid chromotography of tricyclic antidepressants
AU - van den Berg, J.H.M.
AU - de Ruwe, H.J.J.M.
AU - Deelder, R.S.
AU - Plomp, Th.A.
PY - 1977
Y1 - 1977
N2 - A column liquid chromatographic system for the analysis of tricyclic antidepressants in serum is described. A high separation efficiency can be obtained with a mixture of ethyl acetate, n-hexane and methylamine as eluent on a silica gel column. The retention is easily regulated by varying the concentration of n-hexane, the modifier methylamine and the water content of the ethyl acetate.
Examples are given of separations of test mixtures of tricyclic antidepressants and of some of these drugs in serum. UV detection permits determinations down to the 10-ng level in serum.
AB - A column liquid chromatographic system for the analysis of tricyclic antidepressants in serum is described. A high separation efficiency can be obtained with a mixture of ethyl acetate, n-hexane and methylamine as eluent on a silica gel column. The retention is easily regulated by varying the concentration of n-hexane, the modifier methylamine and the water content of the ethyl acetate.
Examples are given of separations of test mixtures of tricyclic antidepressants and of some of these drugs in serum. UV detection permits determinations down to the 10-ng level in serum.
U2 - 10.1016/S0021-9673(00)94978-1
DO - 10.1016/S0021-9673(00)94978-1
M3 - Article
SN - 0021-9673
VL - 138
SP - 431
EP - 436
JO - Journal of Chromatography
JF - Journal of Chromatography
IS - 2
ER -