Collagen mineralization with lepidocrocite: Via Fe(OH)2addition

Bernette M. Oosterlaken (Corresponding author), Mark M.J. van Rijt, Heiner Friedrich, Gijsbertus de With (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

2 Citaten (Scopus)
46 Downloads (Pure)


The mineralization of collagen in vitro has been extensively investigated for hydroxyapatite, silica, calcium carbonate and lepidocrocite (γ-FeOOH). Henceforth, it is interesting to investigate whether collagen also could serve as a generic mineralization template for other minerals, like magnetite. To this end, and inspired by the partial oxidation approach, first a ferrous hydroxide (Fe(OH)2) intermediate is synthesized via the titration of base to a solution of Fe2+. Subsequently, the Fe(OH)2 is mixed with collagen fibrils and poly(aspartic acid) is added to promote the formation of intrafibrillar crystals. Platelet-shaped lepidocrocite crystals being present throughout the entire thickness of the collagen fibrils can be realized, as was confirmed with electron tomography. The formation of lepidocrocite, which is an Fe3+ compound, is hypothesized to be induced via oxidation of the Fe2+ species and, therefore, the oxygen concentration during titration, TEM sample preparation and drying of TEM samples are investigated. Although the reaction is sensitive to small changes in experimental conditions, highly mineralized collagen fibers can be realized. 

Originele taal-2Engels
Pagina's (van-tot)1211-1217
Aantal pagina's7
Nummer van het tijdschrift6
StatusGepubliceerd - 14 feb. 2022

Bibliografische nota

Publisher Copyright:
© The Royal Society of Chemistry.


Duik in de onderzoeksthema's van 'Collagen mineralization with lepidocrocite: Via Fe(OH)2addition'. Samen vormen ze een unieke vingerafdruk.

Citeer dit