Co(III)EDTA as extra-cellular marker in μPIXE-analysis of rat cardiomyocytes

J.A. Quaedackers, R.M.G.J. Queens, P.H.A. Mutsaers, J.J.M. de Goeij, M.J.A. Voigt, de, G.J. Vusse, van der

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    In previous studies no clear difference was found between the intra- and extra-cellular compartment in nuclear microprobe elemental distribution maps of freeze-dried cryo sections of heart tissue. Probably due to artefacts during the preparation of these samples, the intra-cellular and the extra-cellular content of elements are mixed up. In this article a method, using NaCo(III)EDTA as an extra-cellular marker, was applied to deconvolute the total ion content in an extra- and intra-cellular contribution. This method was both applied to normoxic heart tissue and low-flow ischemic heart tissue. Intra-cellular ion concentrations calculated from the corrected ion contents of the normoxic tissue agrees well with literature values. Moreover a clear elevation of the intra-cellular sodium and chlorine concentration was found in low-flow ischemic tissue.
    Originele taal-2Engels
    Pagina's (van-tot)169-174
    Aantal pagina's6
    TijdschriftNuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
    Nummer van het tijdschrift1-4
    StatusGepubliceerd - 1998

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