Choice of capping group in tripeptide hydrogels influences viability in the three-dimensional cell culture of tumor spheroids

Jonathan P. Wojciechowski; Adam D. Martin; Alexander F. Mason; Christopher M. Fife; Sharon M. Sagnella; Maria Kavallaris; Pall Thordarson

doi:10.1002/cplu.201600464

Choice of capping group in tripeptide hydrogels influences viability in the three-dimensional cell culture of tumor spheroids

Jonathan P. Wojciechowski, Adam D. Martin, Alexander F. Mason, Christopher M. Fife, Sharon M. Sagnella, Maria Kavallaris, Pall Thordarson

Onderzoeksoutput: Bijdrage aan tijdschrift › Tijdschriftartikel › Academic › peer review

21 Citaten (Scopus)

Samenvatting

Two peptide-derived low-molecular-weight gelators bearing different capping groups, 9-fluorenylmethyloxycarbonyl (Fmoc) and phenothiazine, were synthesized and their gel networks were characterized. The variation of the N-terminal capping group affects the viability of these hydrogels as a three-dimensional cell culture for multicellular tumor spheroids. These results indicate that the phenothiazine capping group is a more biocompatible alternative to the widely used Fmoc moiety.

Originele taal-2	Engels
Pagina's (van-tot)	383-389
Aantal pagina's	7
Tijdschrift	ChemPlusChem
Volume	82
Nummer van het tijdschrift	3
DOI's	https://doi.org/10.1002/cplu.201600464
Status	Gepubliceerd - 1 mrt. 2017
Extern gepubliceerd	Ja

Financiering

We thank the Mark Wainwright Analytical Centre (UNSW) for access to instruments. We acknowledge the Australian Research Council for an ARC Centre of Excellence grant (CE14010036) to P.T and M.K, a Discovery Project grant (DP130101512), and a Future Fellowship (FT120100101) to P.T, and the National Health and Medical Research Council for a Senior Research Fellowship to M. K. and Dementia Research Development Fellowship (APP1106751) to A.D.M.

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title = "Choice of capping group in tripeptide hydrogels influences viability in the three-dimensional cell culture of tumor spheroids",

abstract = "Two peptide-derived low-molecular-weight gelators bearing different capping groups, 9-fluorenylmethyloxycarbonyl (Fmoc) and phenothiazine, were synthesized and their gel networks were characterized. The variation of the N-terminal capping group affects the viability of these hydrogels as a three-dimensional cell culture for multicellular tumor spheroids. These results indicate that the phenothiazine capping group is a more biocompatible alternative to the widely used Fmoc moiety.",

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AU - Wojciechowski, Jonathan P.

AU - Martin, Adam D.

AU - Mason, Alexander F.

AU - Fife, Christopher M.

AU - Sagnella, Sharon M.

AU - Kavallaris, Maria

AU - Thordarson, Pall

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AB - Two peptide-derived low-molecular-weight gelators bearing different capping groups, 9-fluorenylmethyloxycarbonyl (Fmoc) and phenothiazine, were synthesized and their gel networks were characterized. The variation of the N-terminal capping group affects the viability of these hydrogels as a three-dimensional cell culture for multicellular tumor spheroids. These results indicate that the phenothiazine capping group is a more biocompatible alternative to the widely used Fmoc moiety.

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KW - gels

KW - peptides

KW - self-assembly

KW - tumor spheroids

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Choice of capping group in tripeptide hydrogels influences viability in the three-dimensional cell culture of tumor spheroids

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