Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system

Guansheng Du; Jaap M.J. den Toonder; Qun Fang

Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system

Guansheng Du, Jaap M.J. den Toonder, Qun Fang

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › Conferentiebijdrage › Academic › peer review

Samenvatting

We proposed an integrated and robust microfluidic platform to perform cell-based schedule dependent drug combination screening. Multiple manipulations of cells in droplets, including cell dispensing, culture, drug stimulation and viability test were performed based on a 2D microfluidic droplet array. The screening of flavopiridol (Fla) and 5-fluorouracil (5Flu) in different sequence was carried out in 342 addressable droplets, with a consumption of ∼3 μg drug for each experiment.

Originele taal-2	Engels
Titel	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Plaats van productie	Washington
Uitgeverij	Royal Society of Chemistry
Pagina's	965-967
Aantal pagina's	3
ISBN van geprinte versie	9780979806452
Status	Gepubliceerd - 1 nov. 2012
Evenement	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duur: 28 okt. 2012 → 1 nov. 2012 Congresnummer: 16

Congres

Congres	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Verkorte titel	MicroTAS 2012
Land/Regio	Japan
Stad	Okinawa
Periode	28/10/12 → 1/11/12
Ander	Poster presented at the The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Okinawa, Japan, October 28 - November 1, 2012

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title = "Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system",

abstract = "We proposed an integrated and robust microfluidic platform to perform cell-based schedule dependent drug combination screening. Multiple manipulations of cells in droplets, including cell dispensing, culture, drug stimulation and viability test were performed based on a 2D microfluidic droplet array. The screening of flavopiridol (Fla) and 5-fluorouracil (5Flu) in different sequence was carried out in 342 addressable droplets, with a consumption of ∼3 μg drug for each experiment.",

keywords = "Cell, Drug combination, High-throughput, Microfluidic droplet",

author = "Guansheng Du and {den Toonder}, {Jaap M.J.} and Qun Fang",

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Du, G, den Toonder, JMJ & Fang, Q 2012, Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Royal Society of Chemistry, Washington, blz. 965-967, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 28/10/12.

Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system. / Du, Guansheng; den Toonder, Jaap M.J.; Fang, Qun.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Washington: Royal Society of Chemistry, 2012. blz. 965-967.

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › Conferentiebijdrage › Academic › peer review

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AU - Du, Guansheng

AU - den Toonder, Jaap M.J.

AU - Fang, Qun

N1 - Conference code: 16

PY - 2012/11/1

Y1 - 2012/11/1

N2 - We proposed an integrated and robust microfluidic platform to perform cell-based schedule dependent drug combination screening. Multiple manipulations of cells in droplets, including cell dispensing, culture, drug stimulation and viability test were performed based on a 2D microfluidic droplet array. The screening of flavopiridol (Fla) and 5-fluorouracil (5Flu) in different sequence was carried out in 342 addressable droplets, with a consumption of ∼3 μg drug for each experiment.

AB - We proposed an integrated and robust microfluidic platform to perform cell-based schedule dependent drug combination screening. Multiple manipulations of cells in droplets, including cell dispensing, culture, drug stimulation and viability test were performed based on a 2D microfluidic droplet array. The screening of flavopiridol (Fla) and 5-fluorouracil (5Flu) in different sequence was carried out in 342 addressable droplets, with a consumption of ∼3 μg drug for each experiment.

KW - Cell

KW - Drug combination

KW - High-throughput

KW - Microfluidic droplet

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M3 - Conference contribution

AN - SCOPUS:84901776498

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BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

PB - Royal Society of Chemistry

CY - Washington

T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Y2 - 28 October 2012 through 1 November 2012

ER -

Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system

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