Cancer stem cell migration in an oxygen gradient characterized using a microfluidic device

Jelle J.F. Sleeboom; Cecilia M. Sahlgren; Jaap M.J. Den Toonder

Cancer stem cell migration in an oxygen gradient characterized using a microfluidic device

Jelle J.F. Sleeboom, Cecilia M. Sahlgren, Jaap M.J. Den Toonder

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › Conferentiebijdrage › Academic › peer review

Samenvatting

Recently, cancer stem cells (CSCs) have been proposed as the source of metastases, yet only little is known about the migration of CSCs in the tumor microenvironment (TME). The presence of oxygen gradients in the TME has been linked to directional migration of breast cancer cells. Here, we present a method to study the effect of oxygen gradients on breast CSC migration, using a PDMS microfluidic chip. We demonstrate our capability to perform single cell tracking in a stable gradient. Surprisingly, our results indicate a migration direction opposite to the direction reported in a previous study.

Originele taal-2	Engels
Titel	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Uitgeverij	Chemical and Biological Microsystems Society
Pagina's	1517-1520
Aantal pagina's	4
Volume	3
ISBN van elektronische versie	9781510897571
Status	Gepubliceerd - 2018
Evenement	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan Duur: 11 nov 2018 → 15 nov 2018

Congres

Congres	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Land	Taiwan
Stad	Kaohsiung
Periode	11/11/18 → 15/11/18

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Citeer dit

@inproceedings{01d866c453af49c8846a6bb7996a3776,

title = "Cancer stem cell migration in an oxygen gradient characterized using a microfluidic device",

abstract = "Recently, cancer stem cells (CSCs) have been proposed as the source of metastases, yet only little is known about the migration of CSCs in the tumor microenvironment (TME). The presence of oxygen gradients in the TME has been linked to directional migration of breast cancer cells. Here, we present a method to study the effect of oxygen gradients on breast CSC migration, using a PDMS microfluidic chip. We demonstrate our capability to perform single cell tracking in a stable gradient. Surprisingly, our results indicate a migration direction opposite to the direction reported in a previous study.",

keywords = "Cancer stem cells, Metastasis, Microfluidics, Migration, Oxygen gradient, Tumor microenvironment",

author = "Sleeboom, {Jelle J.F.} and Sahlgren, {Cecilia M.} and {Den Toonder}, {Jaap M.J.}",

year = "2018",

language = "English",

volume = "3",

pages = "1517--1520",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

publisher = "Chemical and Biological Microsystems Society",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

}

Sleeboom, JJF , Sahlgren, CM & Den Toonder, JMJ 2018, Cancer stem cell migration in an oxygen gradient characterized using a microfluidic device. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. vol. 3, Chemical and Biological Microsystems Society, blz. 1517-1520, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, Taiwan, 11/11/18.

Cancer stem cell migration in an oxygen gradient characterized using a microfluidic device. / Sleeboom, Jelle J.F.; Sahlgren, Cecilia M.; Den Toonder, Jaap M.J.

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Vol. 3 Chemical and Biological Microsystems Society, 2018. blz. 1517-1520.

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › Conferentiebijdrage › Academic › peer review

TY - GEN

T1 - Cancer stem cell migration in an oxygen gradient characterized using a microfluidic device

AU - Sleeboom, Jelle J.F.

AU - Sahlgren, Cecilia M.

AU - Den Toonder, Jaap M.J.

PY - 2018

Y1 - 2018

N2 - Recently, cancer stem cells (CSCs) have been proposed as the source of metastases, yet only little is known about the migration of CSCs in the tumor microenvironment (TME). The presence of oxygen gradients in the TME has been linked to directional migration of breast cancer cells. Here, we present a method to study the effect of oxygen gradients on breast CSC migration, using a PDMS microfluidic chip. We demonstrate our capability to perform single cell tracking in a stable gradient. Surprisingly, our results indicate a migration direction opposite to the direction reported in a previous study.

AB - Recently, cancer stem cells (CSCs) have been proposed as the source of metastases, yet only little is known about the migration of CSCs in the tumor microenvironment (TME). The presence of oxygen gradients in the TME has been linked to directional migration of breast cancer cells. Here, we present a method to study the effect of oxygen gradients on breast CSC migration, using a PDMS microfluidic chip. We demonstrate our capability to perform single cell tracking in a stable gradient. Surprisingly, our results indicate a migration direction opposite to the direction reported in a previous study.

KW - Cancer stem cells

KW - Metastasis

KW - Microfluidics

KW - Migration

KW - Oxygen gradient

KW - Tumor microenvironment

UR - http://www.scopus.com/inward/record.url?scp=85079819113&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85079819113

VL - 3

SP - 1517

EP - 1520

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

Y2 - 11 November 2018 through 15 November 2018

ER -