Biosensors and preparation thereof

A low-temp. prepn. method for a biosensor device with a layer of reagent on the sensor surface is disclosed. During manufg. biol. interaction between the biosensor substrate and the reagent layer material is reduced, e.g. by cooling the biosensor substrate and depositing the reagent layer on the cooled biosensor substrate. Alternatively, the biol. interaction between the biosensor substrate and the reagent layer material may also be reduced by providing a sepn. layer. Furthermore, the method comprises, after depositing of the reagent layer, at least maintaining the biol. interaction until the reagent layer is substantially mech. stable. The method reduces or even avoids interaction between reagent and biol. or biochem. active moieties present on the substrate surface. The latter nevertheless allows to obtain a biosensor device with high reactivity of the reagent layer during operation of the biosensor. Biosensors thus obtained allow for fast assays, low reagent use, multiplexing, and small sample vol. [on SciFinder (R)]