TY - JOUR
T1 - Anchoring Supramolecular Polymers to Human Red Blood Cells by Combining Dynamic Covalent and Non-Covalent Chemistries
AU - Morgese, Giulia
AU - de Waal, Bas F.M.
AU - Varela-Aramburu, Silvia
AU - Palmans, Anja R.A.
AU - Albertazzi, Lorenzo
AU - Meijer, E.W.
PY - 2020/9/21
Y1 - 2020/9/21
N2 - Understanding cell/material interactions is essential to design functional cell-responsive materials. While the scientific literature abounds with formulations of biomimetic materials, only a fraction of them focused on mechanisms of the molecular interactions between cells and material. To provide new knowledge on the strategies for materials/cell recognition and binding, supramolecular benzene-1,3,5-tricarboxamide copolymers bearing benzoxaborole moieties are anchored on the surface of human erythrocytes via benzoxaborole/sialic-acid binding. This interaction based on both dynamic covalent and non-covalent chemistries is visualized in real time by means of total internal reflection fluorescence microscopy. Exploiting this imaging method, we observe that the functional copolymers specifically interact with the cell surface. An optimal fiber affinity towards the cells as a function of benzoxaborole concentration demonstrates the crucial role of multivalency in these cell/material interactions.
AB - Understanding cell/material interactions is essential to design functional cell-responsive materials. While the scientific literature abounds with formulations of biomimetic materials, only a fraction of them focused on mechanisms of the molecular interactions between cells and material. To provide new knowledge on the strategies for materials/cell recognition and binding, supramolecular benzene-1,3,5-tricarboxamide copolymers bearing benzoxaborole moieties are anchored on the surface of human erythrocytes via benzoxaborole/sialic-acid binding. This interaction based on both dynamic covalent and non-covalent chemistries is visualized in real time by means of total internal reflection fluorescence microscopy. Exploiting this imaging method, we observe that the functional copolymers specifically interact with the cell surface. An optimal fiber affinity towards the cells as a function of benzoxaborole concentration demonstrates the crucial role of multivalency in these cell/material interactions.
KW - boronic acid
KW - cell/material interactions
KW - multivalency
KW - red blood cells
KW - supramolecular polymers
UR - http://www.scopus.com/inward/record.url?scp=85089547765&partnerID=8YFLogxK
U2 - 10.1002/anie.202006381
DO - 10.1002/anie.202006381
M3 - Article
C2 - 32584462
VL - 59
SP - 17229
EP - 17233
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
SN - 0570-0833
IS - 39
ER -