ACDC: Automated Cell Detection and Counting for time-lapse fluorescence microscopy

Leonardo Rundo, Andrea Tangherloni, Darren Tyson, Riccardo Betta, Carmelo Militello, Simone Spolaor, Marco S. Nobile, Daniela Besozzi, Alex L.R. Lubbock, Vito Quaranta, Giancarlo Mauri, Carlos F. Lopez (Corresponding author), Paolo Cazzaniga (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

Samenvatting

Advances in microscopy imaging technologies have enabled the visualization of live-cell dynamic processes using time-lapse microscopy imaging. However, modern methods exhibit several limitations related to the training phases and to time constraints, hindering their application in the laboratory practice. In this work, we present a novel method, named Automated Cell Detection and Counting (ACDC), designed for activity detection of fluorescent labeled cell nuclei in time-lapse microscopy. ACDC overcomes the limitations of the literature methods, by first applying bilateral filtering on the original image to smooth the input cell images while preserving edge sharpness, and then by exploiting the watershed transform and morphological filtering. Moreover, ACDC represents a feasible solution for the laboratory practice, as it can leverage multi-core architectures in computer clusters to efficiently handle large-scale imaging datasets. ACDC was tested on two distinct cell imaging datasets to assess its accuracy and effectiveness on images with different characteristics, allowing us to achieve an accurate cell-count and nuclei segmentation, without relying on large-scale annotated datasets.
Originele taal-2Engels
Artikelnummer6187
Aantal pagina's22
TijdschriftApplied Sciences
Volume10
Nummer van het tijdschrift18
DOI's
StatusGepubliceerd - 1 sep 2020

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    Rundo, L., Tangherloni, A., Tyson, D., Betta, R., Militello, C., Spolaor, S., Nobile, M. S., Besozzi, D., Lubbock, A. L. R., Quaranta, V., Mauri, G., Lopez, C. F., & Cazzaniga, P. (2020). ACDC: Automated Cell Detection and Counting for time-lapse fluorescence microscopy. Applied Sciences, 10(18), [6187]. https://doi.org/10.1101/2020.07.14.202804v1, https://doi.org/10.3390/app10186187