Accurate assessment of in situ isometric contractile properties of hindlimb plantar and dorsal flexor muscle complex of intact mice

M. Gorselink, M.R. Drost, J. Louw, de, P.J.B. Willems, P.C.J.N. Rosielle, J.D. Janssen, G.J. Vusse, van der

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An isometric torque sensor for measuring in situ contractions of plantar or dorsal flexors of intact mouse hindlimb has been developed and evaluated. With this device, muscle torque can be accurately measured within the range of -14 mN·m to +14 mN·m. Special attention was paid to fixation of the mouse hindlimb to the measurement device. Halothane-anaesthetized Swiss wild-type mice were positioned on the thermostatic measurement platform, and fixated with a hip and foot fixation system. The novel fixation unit was evaluated by measuring knee and ankle displacements during a contraction. A mathematical muscle model was used to quantify the effects of these displacements on the contractile parameters. Measured ankle and knee displacement, due to non-absolute fixation, resulted in a calculated muscle fibre shortening of 2.5%. Simulations of a contraction with this degree of fibre shortening, using the mathematical muscle model, showed only minor effects on maximal torque generation and the temporal parameters (half-relaxation time and 10-50% rise time). Furthermore, we showed that muscle torque in our set-up is hardly affected by eccentricity between ankle and measurement axis. Measured tetanic muscle torques of intact dorsal and plantar flexors were 3.2-0.4 mN·m and 11.8-1.6 mN·m, respectively. The half-relaxation time of plantar flexors was significantly higher than that of dorsal flexors (12.9-2.7 ms versus 8.8-1.2 ms), whereas the 10-50% rise time was longer in plantar (14.9-0.6 ms) than in dorsal (11.8-2.0 ms) flexors.
Originele taal-2Engels
Pagina's (van-tot)665-670
TijdschriftPflügers Archiv : European Journal of Physiology
Volume439
Nummer van het tijdschrift5
DOI's
StatusGepubliceerd - 2000

Vingerafdruk

Hindlimb
Torque
Muscle
Muscles
Ankle
Relaxation time
Knee
Theoretical Models
Equipment and Supplies
Fibers
Halothane
Hip
Foot
Sensors

Citeer dit

@article{57b20c20c6074d879ea29fa95b01363d,
title = "Accurate assessment of in situ isometric contractile properties of hindlimb plantar and dorsal flexor muscle complex of intact mice",
abstract = "An isometric torque sensor for measuring in situ contractions of plantar or dorsal flexors of intact mouse hindlimb has been developed and evaluated. With this device, muscle torque can be accurately measured within the range of -14 mN·m to +14 mN·m. Special attention was paid to fixation of the mouse hindlimb to the measurement device. Halothane-anaesthetized Swiss wild-type mice were positioned on the thermostatic measurement platform, and fixated with a hip and foot fixation system. The novel fixation unit was evaluated by measuring knee and ankle displacements during a contraction. A mathematical muscle model was used to quantify the effects of these displacements on the contractile parameters. Measured ankle and knee displacement, due to non-absolute fixation, resulted in a calculated muscle fibre shortening of 2.5{\%}. Simulations of a contraction with this degree of fibre shortening, using the mathematical muscle model, showed only minor effects on maximal torque generation and the temporal parameters (half-relaxation time and 10-50{\%} rise time). Furthermore, we showed that muscle torque in our set-up is hardly affected by eccentricity between ankle and measurement axis. Measured tetanic muscle torques of intact dorsal and plantar flexors were 3.2-0.4 mN·m and 11.8-1.6 mN·m, respectively. The half-relaxation time of plantar flexors was significantly higher than that of dorsal flexors (12.9-2.7 ms versus 8.8-1.2 ms), whereas the 10-50{\%} rise time was longer in plantar (14.9-0.6 ms) than in dorsal (11.8-2.0 ms) flexors.",
author = "M. Gorselink and M.R. Drost and {Louw, de}, J. and P.J.B. Willems and P.C.J.N. Rosielle and J.D. Janssen and {Vusse, van der}, G.J.",
year = "2000",
doi = "10.1007/s004240050991",
language = "English",
volume = "439",
pages = "665--670",
journal = "Pfl{\"u}gers Archiv : European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer",
number = "5",

}

Accurate assessment of in situ isometric contractile properties of hindlimb plantar and dorsal flexor muscle complex of intact mice. / Gorselink, M.; Drost, M.R.; Louw, de, J.; Willems, P.J.B.; Rosielle, P.C.J.N.; Janssen, J.D.; Vusse, van der, G.J.

In: Pflügers Archiv : European Journal of Physiology, Vol. 439, Nr. 5, 2000, blz. 665-670.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

TY - JOUR

T1 - Accurate assessment of in situ isometric contractile properties of hindlimb plantar and dorsal flexor muscle complex of intact mice

AU - Gorselink, M.

AU - Drost, M.R.

AU - Louw, de, J.

AU - Willems, P.J.B.

AU - Rosielle, P.C.J.N.

AU - Janssen, J.D.

AU - Vusse, van der, G.J.

PY - 2000

Y1 - 2000

N2 - An isometric torque sensor for measuring in situ contractions of plantar or dorsal flexors of intact mouse hindlimb has been developed and evaluated. With this device, muscle torque can be accurately measured within the range of -14 mN·m to +14 mN·m. Special attention was paid to fixation of the mouse hindlimb to the measurement device. Halothane-anaesthetized Swiss wild-type mice were positioned on the thermostatic measurement platform, and fixated with a hip and foot fixation system. The novel fixation unit was evaluated by measuring knee and ankle displacements during a contraction. A mathematical muscle model was used to quantify the effects of these displacements on the contractile parameters. Measured ankle and knee displacement, due to non-absolute fixation, resulted in a calculated muscle fibre shortening of 2.5%. Simulations of a contraction with this degree of fibre shortening, using the mathematical muscle model, showed only minor effects on maximal torque generation and the temporal parameters (half-relaxation time and 10-50% rise time). Furthermore, we showed that muscle torque in our set-up is hardly affected by eccentricity between ankle and measurement axis. Measured tetanic muscle torques of intact dorsal and plantar flexors were 3.2-0.4 mN·m and 11.8-1.6 mN·m, respectively. The half-relaxation time of plantar flexors was significantly higher than that of dorsal flexors (12.9-2.7 ms versus 8.8-1.2 ms), whereas the 10-50% rise time was longer in plantar (14.9-0.6 ms) than in dorsal (11.8-2.0 ms) flexors.

AB - An isometric torque sensor for measuring in situ contractions of plantar or dorsal flexors of intact mouse hindlimb has been developed and evaluated. With this device, muscle torque can be accurately measured within the range of -14 mN·m to +14 mN·m. Special attention was paid to fixation of the mouse hindlimb to the measurement device. Halothane-anaesthetized Swiss wild-type mice were positioned on the thermostatic measurement platform, and fixated with a hip and foot fixation system. The novel fixation unit was evaluated by measuring knee and ankle displacements during a contraction. A mathematical muscle model was used to quantify the effects of these displacements on the contractile parameters. Measured ankle and knee displacement, due to non-absolute fixation, resulted in a calculated muscle fibre shortening of 2.5%. Simulations of a contraction with this degree of fibre shortening, using the mathematical muscle model, showed only minor effects on maximal torque generation and the temporal parameters (half-relaxation time and 10-50% rise time). Furthermore, we showed that muscle torque in our set-up is hardly affected by eccentricity between ankle and measurement axis. Measured tetanic muscle torques of intact dorsal and plantar flexors were 3.2-0.4 mN·m and 11.8-1.6 mN·m, respectively. The half-relaxation time of plantar flexors was significantly higher than that of dorsal flexors (12.9-2.7 ms versus 8.8-1.2 ms), whereas the 10-50% rise time was longer in plantar (14.9-0.6 ms) than in dorsal (11.8-2.0 ms) flexors.

U2 - 10.1007/s004240050991

DO - 10.1007/s004240050991

M3 - Article

VL - 439

SP - 665

EP - 670

JO - Pflügers Archiv : European Journal of Physiology

JF - Pflügers Archiv : European Journal of Physiology

SN - 0031-6768

IS - 5

ER -