A Systems Approach to Study Collagen Type I Self-Assembly: Kinetics and Morphology

María Paula Vena, Laura S. van Hazendonk, Willem van Zyl, Remco Tuinier, Heiner Friedrich (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review


Collagen type I, the main component of the extracellular matrix in vertebrates, is widely used in tissue engineering applications. This is on account that collagen molecules can self-assemble under certain conditions into 3D fibrillar hydrogels. Although there is an extensive body of literature studying collagen self-assembly, there is a lack of systematic understanding on how different experimental factors, such as pH and temperature, and their cumulative effects guide the self-assembly process. In this work, a comprehensive workflow to study the interactive effects of several assembly parameters on the collagen self-assembly process is implemented. This workflow consists of: 1) efficient statistical sampling based on Design of Experiments, 2) high-throughput and automated data collection and 3) automated data analysis. This approach enables to screen several parameters simultaneously and derive a set of mathematical equations that link parameters with the kinetics and morphological aspects of collagen self-assembly, and can be used to design collagen constructs with predefined characteristics.

Originele taal-2Engels
TijdschriftSmall Methods
Nummer van het tijdschriftX
StatusE-publicatie vóór gedrukte publicatie - 17 jan. 2024


M.P.V. and L.S.H. contributed equally to this work. M.P.V. acknowledges financial support from the EU H2020 Marie Sklodowska‐Curie Action project MULTIMAT. The authors thank Dr. Mohammad Moradi for the fruitful discussions on Design of Experiments, Dr. Rob Mesman (Radboud University Nijmegen, The Netherlands) for advice on sample preparation for SEM and Dr. Wiggert Altenburg (TU/e, The Netherlands) for helping with access to the Tecan Spark plate reader.

H2020 Marie Skłodowska-Curie Actions


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