A stirring-related aspecific ion-exclusion effect in an ultrafiltration-aided protein-ligand binding study

J.P.M. Wielders

doi:10.1016/0003-2697(80)90629-6

A stirring-related aspecific ion-exclusion effect in an ultrafiltration-aided protein-ligand binding study

J.P.M. Wielders (Corresponding author)

Chemical Engineering and Chemistry

Onderzoeksoutput: Bijdrage aan tijdschrift › Tijdschriftartikel › Academic › peer review

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Samenvatting

The occurrence at 0.1-0.01 m ionic strength of an aspecific ion-exclusion effect at the membrane during ultrafiltrations through Amicon PM 10 and XM 50 membranes is demonstrated. The extent of this phenomenon was found to be related to the shear rate at the membrane surface and to the ionic strength. Concentrations were measured by isotachophoretic analysis. Because of this effect, the use of an internal standard component is strongly recommended in (ultrafiltration-aided) binding studies.

Originele taal-2	Engels
Pagina's (van-tot)	394-399
Aantal pagina's	6
Tijdschrift	Analytical Biochemistry
Volume	103
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1016/0003-2697(80)90629-6
Status	Gepubliceerd - 1 jan. 1980

Financiering

work and the preparation of this paper is acknowledged. The author was supported by a grant from the Netherlands Organisation for the Advancement of Pure Research (Z. W.O.).

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abstract = "The occurrence at 0.1-0.01 m ionic strength of an aspecific ion-exclusion effect at the membrane during ultrafiltrations through Amicon PM 10 and XM 50 membranes is demonstrated. The extent of this phenomenon was found to be related to the shear rate at the membrane surface and to the ionic strength. Concentrations were measured by isotachophoretic analysis. Because of this effect, the use of an internal standard component is strongly recommended in (ultrafiltration-aided) binding studies.",

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N2 - The occurrence at 0.1-0.01 m ionic strength of an aspecific ion-exclusion effect at the membrane during ultrafiltrations through Amicon PM 10 and XM 50 membranes is demonstrated. The extent of this phenomenon was found to be related to the shear rate at the membrane surface and to the ionic strength. Concentrations were measured by isotachophoretic analysis. Because of this effect, the use of an internal standard component is strongly recommended in (ultrafiltration-aided) binding studies.

AB - The occurrence at 0.1-0.01 m ionic strength of an aspecific ion-exclusion effect at the membrane during ultrafiltrations through Amicon PM 10 and XM 50 membranes is demonstrated. The extent of this phenomenon was found to be related to the shear rate at the membrane surface and to the ionic strength. Concentrations were measured by isotachophoretic analysis. Because of this effect, the use of an internal standard component is strongly recommended in (ultrafiltration-aided) binding studies.

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A stirring-related aspecific ion-exclusion effect in an ultrafiltration-aided protein-ligand binding study

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