A stirring-related aspecific ion-exclusion effect in an ultrafiltration-aided protein-ligand binding study

J.P.M. Wielders (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

1 Citaat (Scopus)

Samenvatting

The occurrence at 0.1-0.01 m ionic strength of an aspecific ion-exclusion effect at the membrane during ultrafiltrations through Amicon PM 10 and XM 50 membranes is demonstrated. The extent of this phenomenon was found to be related to the shear rate at the membrane surface and to the ionic strength. Concentrations were measured by isotachophoretic analysis. Because of this effect, the use of an internal standard component is strongly recommended in (ultrafiltration-aided) binding studies.

Originele taal-2Engels
Pagina's (van-tot)394-399
Aantal pagina's6
TijdschriftAnalytical Biochemistry
Volume103
Nummer van het tijdschrift2
DOI's
StatusGepubliceerd - 1 jan 1980

    Vingerafdruk

Citeer dit