A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase

J.H.M. van den Berg, C.R. Mol, R.S. Deelder, J.H.H. Thijssen

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

62 Citaten (Scopus)

Samenvatting

The extraction and subsequent liquid chromatographic analysis of human plasma samples for cortisol is described. Extraction and chromatography are optimized, resulting in a recovery for cortisol of 96% and a detection limit of 1 microgram cortisol in 100 ml plasma. The application of two chemically modified silicas has been evaluated. The specificity of the method was tested by field desorption - mass spectrometry experiments.
Originele taal-2Engels
Pagina's (van-tot)165-172
TijdschriftClinica Chimica Acta
Volume78
DOI's
StatusGepubliceerd - 1977

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