A pipette-tip based method for seeding cells to droplet microfluidic platforms

Nidhi Sinha, Nikita Subedi, Florian Wimmers, Melf Soennichsen, Jurjen Tel (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

5 Citaten (Scopus)
59 Downloads (Pure)

Samenvatting

Amongst various microfluidic platform designs frequently used for cellular analysis, droplet-microfluidics provides a robust tool for isolating and analyzing cells at the single-cell level by eliminating the influence of external factors on the cellular microenvironment. Encapsulation of cells in droplets is dictated by the Poisson distribution as a function of the number of cells present in each droplet and the average number of cells per volume of droplet. Primary cells, especially immune cells, or clinical specimens can be scarce and loss-less encapsulation of cells remains challenging. In this paper, we present a new methodology that uses pipette-tips to load cells to droplet-based microfluidic devices without the significant loss of cells. With various cell types , we demonstrate efficient cell encapsulation in droplets that closely corresponds to the encapsulation efficiency predicted by the Poisson distribution. Our method ensures loss-less loading of cells to microfluidic platforms and can be easily adapted for downstream single cell analysis, e.g., to decode cellular interactions between different cell types.

Originele taal-2Engels
Artikelnummere57848
Aantal pagina's10
TijdschriftJournal of Visualized Experiments (JoVE)
Nummer van het tijdschrift144
DOI's
StatusGepubliceerd - 11 feb 2019

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