A covalent and cleavable antibody-DNA conjugation strategy for sensitive protein detection via immuno-PCR

J.A.G.L. van Buggenum, J.P. Gerlach, S. Eising, L. Schoonen, R.A.P.M. van Eijl, S.E.J. Tanis, M. Hogeweg, N.C. Hubner, J.C.M. van Hest, K.M. Bonger, K.W. (Klaas) Mulder

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Samenvatting

Immuno-PCR combines specific antibody-based protein detection with the sensitivity of PCR-based quantification through the use of antibody-DNA conjugates. The production of such conjugates depends on the availability of quick and efficient conjugation strategies for the two biomolecules. Here, we present an approach to produce cleavable antibody-DNA conjugates, employing the fast kinetics of the inverse electron-demand Diels-Alder reaction between tetrazine and trans-cyclooctene (TCO). Our strategy consists of three steps. First, antibodies are functionalized with chemically cleavable NHS-s-s-tetrazine. Subsequently, double-stranded DNA is functionalized with TCO by enzymatic addition of N3-dATP and coupling to trans-Cyclooctene-PEG12-Dibenzocyclooctyne (TCO-PEG12-DBCO). Finally, conjugates are quickly and efficiently obtained by mixing the functionalized antibodies and dsDNA at low molar ratios of 1:2. In addition, introduction of a chemically cleavable disulphide linker facilitates release and sensitive detection of the dsDNA after immuno-staining. We show specific and sensitive protein detection in immuno-PCR for human epidermal stem cell markers, ITGA6 and ITGB1, and the differentiation marker Transglutaminase 1 (TGM1). We anticipate that the production of chemically cleavable antibody-DNA conjugates will provide a solid basis for the development of multiplexed immuno-PCR experiments and immuno-sequencing methodologies.
Originele taal-2Engels
Artikelnummer22675
TijdschriftScientific Reports
Volume6
DOI's
StatusGepubliceerd - 7 mrt 2016
Extern gepubliceerdJa

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