A biomimetic model for mineralization of type-I collagen fibrils

Shasha Yao, Yifei Xu, Changyu Shao, Fabio Nudelman, Nico A.J.M. Sommerdijk, Ruikang Tang

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureHoofdstukAcademicpeer review

Uittreksel

The bone and dentin mainly consist of type-I collagen fibrils mineralized by hydroxyapatite (HAP) nanocrystals. In vitro biomimetic models based on self-assembled collagen fibrils have been widely used in studying the mineralization mechanism of type-I collagen. In this chapter, the protocol we used to build a biomimetic model for the mechanistic study of type-I collagen mineralization is described. Type-I collagen extracted from rat tail tendon or horse tendon is self-assembled into fibrils and mineralized by HAP in vitro. The mineralization process is monitored by cryoTEM in combination with two-dimensional (2D) and three-dimensional (3D) stochastic optical reconstruction microscopy (STORM), which enables in situ and high-resolution visualization of the process.

Originele taal-2Engels
TitelCollagen
SubtitelMethods and protocols
RedacteurenIrit Sagi, Nikolaos A. Afratis
UitgeverijSpringer
Pagina's39-54
Aantal pagina's16
ISBN van elektronische versie978-1-4939-9095-5
ISBN van geprinte versie978-1-2939-9094-8
DOI's
StatusGepubliceerd - 1 jan 2019

Publicatie series

NaamMethods in Molecular Biology
UitgeverijHumana Press
Volume1944
ISSN van geprinte versie1064-3745

Vingerafdruk

Biomimetics
Collagen Type I
Durapatite
Tendons
Dentin
Nanoparticles
Horses
Tail
Microscopy
Collagen
Bone and Bones
In Vitro Techniques

Citeer dit

Yao, S., Xu, Y., Shao, C., Nudelman, F., Sommerdijk, N. A. J. M., & Tang, R. (2019). A biomimetic model for mineralization of type-I collagen fibrils. In I. Sagi, & N. A. Afratis (editors), Collagen: Methods and protocols (blz. 39-54). (Methods in Molecular Biology; Vol. 1944). Springer. https://doi.org/10.1007/978-1-4939-9095-5_4
Yao, Shasha ; Xu, Yifei ; Shao, Changyu ; Nudelman, Fabio ; Sommerdijk, Nico A.J.M. ; Tang, Ruikang. / A biomimetic model for mineralization of type-I collagen fibrils. Collagen: Methods and protocols. redacteur / Irit Sagi ; Nikolaos A. Afratis. Springer, 2019. blz. 39-54 (Methods in Molecular Biology).
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abstract = "The bone and dentin mainly consist of type-I collagen fibrils mineralized by hydroxyapatite (HAP) nanocrystals. In vitro biomimetic models based on self-assembled collagen fibrils have been widely used in studying the mineralization mechanism of type-I collagen. In this chapter, the protocol we used to build a biomimetic model for the mechanistic study of type-I collagen mineralization is described. Type-I collagen extracted from rat tail tendon or horse tendon is self-assembled into fibrils and mineralized by HAP in vitro. The mineralization process is monitored by cryoTEM in combination with two-dimensional (2D) and three-dimensional (3D) stochastic optical reconstruction microscopy (STORM), which enables in situ and high-resolution visualization of the process.",
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Yao, S, Xu, Y, Shao, C, Nudelman, F, Sommerdijk, NAJM & Tang, R 2019, A biomimetic model for mineralization of type-I collagen fibrils. in I Sagi & NA Afratis (redactie), Collagen: Methods and protocols. Methods in Molecular Biology, vol. 1944, Springer, blz. 39-54. https://doi.org/10.1007/978-1-4939-9095-5_4

A biomimetic model for mineralization of type-I collagen fibrils. / Yao, Shasha; Xu, Yifei; Shao, Changyu; Nudelman, Fabio; Sommerdijk, Nico A.J.M.; Tang, Ruikang.

Collagen: Methods and protocols. redactie / Irit Sagi; Nikolaos A. Afratis. Springer, 2019. blz. 39-54 (Methods in Molecular Biology; Vol. 1944).

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureHoofdstukAcademicpeer review

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Yao S, Xu Y, Shao C, Nudelman F, Sommerdijk NAJM, Tang R. A biomimetic model for mineralization of type-I collagen fibrils. In Sagi I, Afratis NA, redacteurs, Collagen: Methods and protocols. Springer. 2019. blz. 39-54. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9095-5_4