3D Printed Magneto-Active Microfiber Scaffolds for Remote Stimulation and Guided Organization of 3D In Vitro Skeletal Muscle Models

Gerardo Cedillo-Servin, Ouafa Dahri, João Meneses, Joost van Duijn, Harrison Moon, Fanny Sage, Joana Silva, André Pereira, Fernão D. Magalhães, Jos Malda, Niels Geijsen, Artur M. Pinto, Miguel Castilho (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

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Samenvatting

This work reports the rational design and fabrication of magneto-active microfiber meshes with controlled hexagonal microstructures via melt electrowriting (MEW) of a magnetized polycaprolactone-based composite. In situ iron oxide nanoparticle deposition on oxidized graphene yields homogeneously dispersed magnetic particles with sizes above 0.5 µm and low aspect ratio, preventing cellular internalization and toxicity. With these fillers, homogeneous magnetic composites with high magnetic content (up to 20 weight %) are obtained and processed in a solvent-free manner for the first time. MEW of magnetic composites enabled the creation of skeletal muscle-inspired design of hexagonal scaffolds with tunable fiber diameter, reconfigurable modularity, and zonal distribution of magneto-active and nonactive material, with elastic tensile deformability. External magnetic fields below 300 mT are sufficient to trigger out-of-plane reversible deformation. In vitro culture of C2C12 myoblasts on three-dimensional (3D) Matrigel/collagen/MEW scaffolds showed that microfibers guided the formation of 3D myotube architectures, and the presence of magnetic particles does not significantly affect viability or differentiation rates after 8 days. Centimeter-sized skeletal muscle constructs allowed for reversible, continued, and dynamic magneto-mechanical stimulation. Overall, these innovative microfiber scaffolds provide magnetically deformable platforms suitable for dynamic culture of skeletal muscle, offering potential for in vitro disease modeling.

Originele taal-2Engels
Artikelnummer2307178
Aantal pagina's15
TijdschriftSmall
Volume20
Nummer van het tijdschrift12
Vroegere onlinedatum10 nov. 2023
DOI's
StatusGepubliceerd - 22 mrt. 2024

Financiering

G.C‐S. and O.D. contributed equally to this work. G.C‐S., J.M., and M.C. acknowledge financial support from the Netherlands Organization for Scientific Research (NWO) through the Gravitation Program “Materials Driven Regeneration” (024.003.013) and the European Union Horizon 2020 program through project BRAV3 (874827). M.C. acknowledges the financial support from the NWO through project RePrint (OCENW.XS5.161). O.D., F.G.S., and N.G. acknowledge the financial support from the Novo Nordisk Foundation (NNF21CC0073729), FSHDglobal, and the Stichting Utrecht Singelswim. J.M., F.D.M., and A.M.P. acknowledge financial support from the Portuguese Foundation for Science and Technology (FCT) / Ministry for Science, Technology, and Higher Education (MCTES PIDDAC) through projects ALiCE (LA/P/0045/2020), LEPABE (UIDB/00511/2020 and UIDP/00511/2020), and PhotoRect (FCT 2022‐04494‐PTDC), and the Institute for Research and Innovation in Health i3S (UIDB/04293/2020); through the UT Austin PT Program (project UTAP‐EXPL/NPN/0044/2021); from FEDER funds through the COMPETE 2020–Operational Programme for Competitiveness and Internationalisation, Portugal; and from the Norte Portugal Regional Operational Programme (NORTE) within the Portugal 2020 Partnership Agreement of the European Regional Development Fund through project 2SMART (NORTE‐01‐0145‐FEDER‐000054). A.M.P. acknowledges financial support from the F.C.T. through the Scientific Employment Stimulus (Individual Call, CEECIND/03908/2017).

FinanciersFinanciernummer
European Union’s Horizon Europe research and innovation programmeOCENW.XS5.161, 874827
Institute for Research and Innovation in Health i3SUIDB/04293/2020
Ministry for Science, Technology, and Higher Education
Norte Portugal Regional Operational Programme
Scientific Employment StimulusCEECIND/03908/2017
Stichting Utrecht Singelswim
UT Austin PT ProgramUTAP‐EXPL/NPN/0044/2021
Fundacao para a Ciencia e Tecnologia (FCT)
Nederlandse Organisatie voor Wetenschappelijk Onderzoek024.003.013
Ministério da Ciência, Tecnologia e Ensino SuperiorUIDB/00511/2020, FCT 2022‐04494‐PTDC, LA/P/0045/2020, UIDP/00511/2020
European Regional Development Fund2SMART (NORTE‐01‐0145‐FEDER‐000054
Novo Nordisk FondenNNF21CC0073729
Programa Operacional Temático Factores de Competitividade

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