• 5316 Citaten
20052020
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Persoonlijk profiel

Research profile

Tom de Greef is Associate Professor of Synthetic Biology in the department of Biomedical Engineering. Work in the Synthetic Biology group (7 PhD students, 2 post-docs) is directed at the bottom-up construction of basic cellular functions from well-characterized biological components, and the development of novel biological computing devices that can enhance signal-processing capabilities of natural and synthetic cells. This involves the design and construction of integrated genetic, protein or DNA-based circuits, either in a cell-free environment or in living cells, capable of autonomously performing useful functions. To achieve this goal, a multidisciplinary approach is employed that combines elements from DNA/RNA nanotechnology, genetic engineering, microengineering and the modelling and simulation of biomolecular networks. Ultimately, advances in synthetic biology will allow the development of functional living and hybrid technologies such as biological robots, synthetic cells or augmented natural cells, that can be interfaced with the human body to detect disease biomarkers and allow autonomous, closed-loop therapeutic functions. 

Academic background

Tom de Greef studied Biomedical Engineering at Eindhoven University of Technology (TU/e, the Netherlands), where he received his MSc degree cum laude in 2004. He then started his PhD research at the TU/e department of Chemical Engineering and Chemistry, where he graduated in 2008 on a thesis on novel polymeric materials based on quadruple hydrogen-bonding motifs, supervised by professors E. W. (Bert) Meijer and Rint Sijbesma. He subsequently moved to the Computational Biology group at the TU/e department of Biomedical Engineering (TU/e) headed by Prof. Peter Hilbers, for postdoctoral research on self-assembling systems from a computational perspective. In 2010, he became assistant professor and in 2016 he was promoted to associate professor. In 2013, Tom de Greef was a visiting scholar in the group of Prof. David Weitz at Harvard University (Cambridge, USA), working on protein affinity screening using droplet microfluidics.

In 2012 and 2013 the Netherlands Organization of Scientific Research (NWO) awarded him a VENI grant and an ECHO-STIP grant, respectively. In 2015 he received an ERC starting Grant and in 2016 an NWO VIDI grant. Tom de Greef is a core member of the Institute for Complex Molecular Systems (ICMS), a core member of the Dutch Gravitation program "Functional Molecular Systems" and a junior faculty member of the Gravitation program "Materials-Driven Regeneration". He received the 2017 Cram Lehn Pedersen prize in supramolecular chemistry, and in 2018 he was awarded a Microsoft PhD scholarship for the development of a DNA computer.

Quote

"When we learn to control the flow of biological information and use it to our advantage, we will reach the pinnacle of the Information Age."

Externe posities

Visiting Scholar, Harvard University

1 apr 201310 okt 2013

Vingerafdruk Duik in de onderzoeksthema's waar Tom F.A. de Greef actief is. Deze onderwerplabels komen voort uit het werk van deze persoon. Samen vormen ze een unieke vingerafdruk.

  • 7 Vergelijkbare profielen
Polymers Chemische stoffen
Self assembly Chemische stoffen
Polymerization Chemische stoffen
Hydrogen bonds Chemische stoffen
DNA Engineering en materiaalwetenschappen
Monomers Chemische stoffen
Pyrimidinones Chemische stoffen
Molecules Chemische stoffen

Netwerk Recente externe samenwerking op landenniveau. Duik in de details door op de stippen te klikken.

Onderzoeksoutput 2005 2020

5 Downloads (Pure)

Proximity-induced caspase-9 activation on a DNA origami-based synthetic apoptosome

Rosier, B., Markvoort, B., Gumi Audenis, B., Roodhuizen, J., den Hamer, A., Brunsveld, L. & de Greef, T. F. A., 6 jan 2020, In : Nature Catalysis. 12 blz.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

Open Access
Apoptosomes
Caspase 9
DNA
Protein Engineering
Multiprotein Complexes
74 Downloads (Pure)

A multilayer microfluidic platform for the conduction of prolonged cell-free gene expression

van der Linden, A., Pieters, P., Yelleswarapu, M., Swank, Z., Huck, W. T. S., Maerkl, S. & de Greef, T., 1 okt 2019, In : Journal of Visualized Experiments (JoVE). 152, 14 blz., e59655.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

Open Access
Bestand
Lab-On-A-Chip Devices
Microfluidics
Transcription
Gene expression
Multilayers
6 Citaties (Scopus)
56 Downloads (Pure)

Cell-free microcompartmentalised transcription-translation for the prototyping of synthetic communication networks

Dubuc, E., Pieters, P. A., van der Linden, A. J., van Hest, J. C. M., Huck, W. T. S. & de Greef, T. F. A., 1 aug 2019, In : Current Opinion in Biotechnology. 58, blz. 72-80 9 blz.

Onderzoeksoutput: Bijdrage aan tijdschriftArtikel recenserenAcademicpeer review

Open Access
Bestand
Transcription
Telecommunication networks
Communication
Cells
Networks (circuits)
15 Downloads (Pure)

Counterion-dependent mechanisms of DNA origami nanostructure stabilization revealed by atomistic molecular simulation

Roodhuizen, J., Hendrikx, P. J. T. M., Hilbers, P. A. J., de Greef, T. & Markvoort, B., 10 sep 2019, In : ACS Nano. 13, 9, blz. 10798-10809 12 blz.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

Open Access
Bestand
helices
Nanostructures
DNA
deoxyribonucleic acid
Stabilization
22 Citaties (Scopus)
6 Downloads (Pure)

DNA-based communication in populations of synthetic protocells

Joesaar, A. H., Yang, S., Bögels, B. W. A., van der Linden, A. J., Pieters, P. A., Kumar, P., Dachau, N., Phillips, A., Mann, S. & de Greef, T. F. A., 1 apr 2019, In : Nature Nanotechnology. 14, 4, blz. 369-378 10 blz.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

DNA
deoxyribonucleic acid
communication
Communication
Networks (circuits)

Prijzen

COST Action CM0703

T.F.A. de Greef (Ontvanger), 2011

Prijs: AndersDiscipline gerelateerdProfessioneel

Cram Lehn Pedersen Prize

T.F.A. de Greef (Ontvanger), 2017

Prijs: AndersDiscipline gerelateerdWetenschappelijk

Supramolecular chemistry

Microsoft PhD Scholarship

T.F.A. de Greef (Ontvanger), 2018

Prijs: AndersBeurzenWetenschappelijk

NWO Veni Award : Folding and aggregation in biohybrid macromolecules

T.F.A. de Greef (Ontvanger), 2012

Prijs: NWOVeniWetenschappelijk

Macromolecules
Self assembly
Agglomeration
Polymers
Mathematical models
Synthetic Biology

Cursussen

iGEM project

1/09/14 → …

Cursus

Inleiding organische chemie

1/09/12 → …

Cursus

Project Computational biology

1/09/1431/08/20

Cursus

Simulatie biochemische systemen

1/09/13 → …

Cursus

Pers/media

THE YOUNG ACADEMY WELCOMES TEN NEW MEMBERS

Tom F.A. de Greef & Liesbeth M.C. Janssen

10/12/19

1 item van Media-aandacht

Pers / media: Vakinhoudelijk commentaar

Young Academy welcomes Liesbeth Janssen

Carlijn V.C. Bouten & Tom F.A. de Greef

10/12/19

1 item van Media-aandacht

Pers / media: Vakinhoudelijk commentaar

Molecular data-storage system encodes information with peptides

Tom F.A. de Greef

1/05/19

1 item van Media-aandacht

Pers / media: Vakinhoudelijk commentaar

Protocells use DNA logic to communicate and compute

Tom F.A. de Greef

4/03/197/03/19

9 items van Media-aandacht

Pers / media: Vakinhoudelijk commentaar

Protocells Utilize DNA Logic to Communicate and Compute

Tom F.A. de Greef

6/03/19

1 item van Media-aandacht

Pers / media: Vakinhoudelijk commentaar

Scriptie

A microfluidic toolbox for supramolecular chemistry and chemical biology

Auteur: Rosier, B., 31 okt 2014

Begeleider: de Greef, T. (Afstudeerdocent 1) & Meijer, E. (Afstudeerdocent 2)

Scriptie/masterproef: Master

Cationic stabilization of DNA origami investigated through atomistic simulations

Auteur: Hendrikx, P., 29 nov 2018

Begeleider: Roodhuizen, J. (Afstudeerdocent 1), Markvoort, B. (Afstudeerdocent 2), de Greef, T. (Afstudeerdocent 2) & Hilbers, P. (Afstudeerdocent 2)

Scriptie/masterproef: Master

Coupling of an enzymatic actuator to an oscillating PEN-based DNA network

Auteur: Nijenhuis, M., 25 jan 2018

Begeleider: Meijer, L. (Afstudeerdocent 1), de Greef, T. (Afstudeerdocent 2) & Hilbers, P. (Afstudeerdocent 2)

Scriptie/masterproef: Master

Creating a non-linear response in dCas9-based gene repression: tuning the sgRNA concentration with the use of enzymatic coupled degradation

Auteur: Uittenbogaard, P., 20 mei 2019

Begeleider: Hilbers, P. (Afstudeerdocent 1), de Greef, T. (Afstudeerdocent 2), Ottmann, C. (Afstudeerdocent 2) & Dubuc, E. (Afstudeerdocent 2)

Scriptie/masterproef: Master

Design of a DNA-based tension probe for the quantification of cellular traction forces at a molecular level

Auteur: van der Putten, C., 25 jan 2018

Begeleider: Kurniawan, N. (Afstudeerdocent 1), de Greef, T. (Afstudeerdocent 2) & Bouten, C. (Afstudeerdocent 2)

Scriptie/masterproef: Master