Uitrustingsdetails
Tekst
Introduction
HPLC is an analytical technique for the separation, identification and quantification of solutes in a solvent.
Principle
Differences in the relative affinities of molecules for the mobile phase and the stationary phase separate a sample into its constituents. The eluent can be a single solvent (isocratic) or a mixture with changing composition (gradient). There are three main operational modes: normal phase - based on polarity; reversed phase - based on hydrophobic interactions and ion exchange - based on electrostatic interactions.
Applications
• Identification and quantification and of solutes and components.
• Micropollutant detection
• Membrane performance evaluation
Each component is associated with a peak characteristic retention time. The concentration is proportional to the integrated peak area.
HPLC is an analytical technique for the separation, identification and quantification of solutes in a solvent.
Principle
Differences in the relative affinities of molecules for the mobile phase and the stationary phase separate a sample into its constituents. The eluent can be a single solvent (isocratic) or a mixture with changing composition (gradient). There are three main operational modes: normal phase - based on polarity; reversed phase - based on hydrophobic interactions and ion exchange - based on electrostatic interactions.
Applications
• Identification and quantification and of solutes and components.
• Micropollutant detection
• Membrane performance evaluation
Each component is associated with a peak characteristic retention time. The concentration is proportional to the integrated peak area.
Details
Naam | 1100 and 1200 Series |
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Fabrikanten | Agilent Technologies |
Naam | HPLC 1200 & 1250 |
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