High Performance Liquid Chromatography (HPLC)

Uitrusting/faciliteit: Uitrusting

    Uitrustingsdetails

    Tekst

    Introduction

    HPLC is an analytical technique for the separation, identification and quantification of solutes in a solvent.

    Principle

    Differences in the relative affinities of molecules for the mobile phase and the stationary phase separate a sample into its constituents. The eluent can be a single solvent (isocratic) or a mixture with changing composition (gradient). There are three main operational modes: normal phase - based on polarity; reversed phase - based on hydrophobic interactions and ion exchange - based on electrostatic interactions.

    Applications

    • Identification and quantification and of solutes and components.
    • Micropollutant detection
    • Membrane performance evaluation

    Each component is associated with a peak characteristic retention time. The concentration is proportional to the integrated peak area.

    Details

    Naam1100 and 1200 Series
    FabrikantenAgilent Technologies

    Vingerafdruk Verken de onderzoeksgebieden waarvoor deze uitrusting is gebruikt. Deze labels worden gegenereerd op basis van de gerelateerde outputs. Samen vormen ze een unieke vingerafdruk.