X-ray structure of p38α bound to TAK-715: comparison with three classic inhibitors

R. Azevedo, M. Zeeland, van, H. Raaijmakers, B. Kazemier, J. Vlieg, de, A. Oubrie

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)


The p38a mitogen-activated protein kinase regulates the synthesis of pro-inflammatory cytokines in response to stimulation by a diverse set of stress signals. Various different chemotypes and clinical candidates that inhibit p38a function have been reported over the years. In this publication, the novel structure of p38a cocrystallized with the clinical candidate TAK-715 is reported. Owing to the impact of crystallization conditions on the conformation of protein kinases (and in particular p38a), the structures of complexes of p38a with SB-203580, SCIO-469 and VX-745 have also been determined to enable in-depth comparison of ligand-induced protein conformations. The impact of experimental conditions on p38a–inhibitor complex structures, most importantly soaking versus cocrystallization, is discussed. Analysis of the structures and quantification of the protein–ligand interactions couples ligand-induced protein conformations to the number of interactions and to inhibitor selectivity against the human kinome. This shows that for the design of novel kinase inhibitors, selectivity is best obtained through maximization of the number of interactions throughout the ATP pocket and the exploitation of specific features in the active site. Keywords: p38a;TAK-715;SB-203580;SCIO-469;VX-745;selectivity;soaking;protein kinases;inhibitor design
Original languageEnglish
Pages (from-to)1041-1050
JournalActa Crystallographica, Section D: Biological Crystallography
Issue number8
Publication statusPublished - 2012
Externally publishedYes


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