Visualization of vasculature using a hand-held photoacoustic probe: phantom and in vivo validation

H.M. Heres, M.Ü. Arabul, M.C.M. Rutten, F.N. van de Vosse, R.G.P. Lopata

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)
506 Downloads (Pure)

Abstract

Assessment of microvasculature and tissue perfusion can provide diagnostic information on local or systemic diseases. Photoacoustic (PA) imaging has strong clinical potential because of its sensitivity to hemoglobin. We used a hand-held PA probe with integrated diode lasers and examined its feasibility and validity in the detection of increasing blood volume and (sub) dermal vascularization. Blood volume detection was tested in custom-made perfusion phantoms. Results showed that an increase of blood volume in a physiological range of 1.3% to 5.4% could be detected. The results were validated with power Doppler sonography. Using a motorized scanning setup, areas of the skin were imaged at relatively short scanning times (<10  s/cm 2 <10  s/cm2 ) with PA. Three-dimensional visualization of these structures was achieved by combining the consecutively acquired cross-sectional images. Images revealed the epidermis and submillimeter vasculature up to depth of 5 mm. The geometries of imaged vasculature were validated with segmentation of the vasculature in high-frequency ultrasound imaging. This study proves the feasibility of PA imaging in its current implementation for the detection of perfusion-related parameters in skin and subdermal tissue and underlines its potential as a diagnostic tool in vascular or dermal pathologies.
Original languageEnglish
Article number041013
Pages (from-to)1-8
Number of pages8
JournalJournal of Biomedical Optics
Volume22
Issue number4
DOIs
Publication statusPublished - Apr 2017

Fingerprint

blood volume
Photoacoustic effect
Visualization
Blood
probes
Imaging techniques
epidermis
Skin
scanning
hemoglobin
pathology
Tissue
Scanning
Ultrasonography
Hemoglobin
Pathology
semiconductor lasers
Semiconductor lasers
Hemoglobins
Ultrasonics

Keywords

  • photoacoustic imaging
  • vasculature
  • validation
  • phantom
  • probe

Cite this

@article{f279dd72f716459397c3dbe6682872b4,
title = "Visualization of vasculature using a hand-held photoacoustic probe: phantom and in vivo validation",
abstract = "Assessment of microvasculature and tissue perfusion can provide diagnostic information on local or systemic diseases. Photoacoustic (PA) imaging has strong clinical potential because of its sensitivity to hemoglobin. We used a hand-held PA probe with integrated diode lasers and examined its feasibility and validity in the detection of increasing blood volume and (sub) dermal vascularization. Blood volume detection was tested in custom-made perfusion phantoms. Results showed that an increase of blood volume in a physiological range of 1.3{\%} to 5.4{\%} could be detected. The results were validated with power Doppler sonography. Using a motorized scanning setup, areas of the skin were imaged at relatively short scanning times (<10  s/cm 2 <10  s/cm2 ) with PA. Three-dimensional visualization of these structures was achieved by combining the consecutively acquired cross-sectional images. Images revealed the epidermis and submillimeter vasculature up to depth of 5 mm. The geometries of imaged vasculature were validated with segmentation of the vasculature in high-frequency ultrasound imaging. This study proves the feasibility of PA imaging in its current implementation for the detection of perfusion-related parameters in skin and subdermal tissue and underlines its potential as a diagnostic tool in vascular or dermal pathologies.",
keywords = "photoacoustic imaging, vasculature, validation, phantom, probe",
author = "H.M. Heres and M.{\"U}. Arabul and M.C.M. Rutten and {van de Vosse}, F.N. and R.G.P. Lopata",
year = "2017",
month = "4",
doi = "10.1117/1.JBO.22.4.041013",
language = "English",
volume = "22",
pages = "1--8",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "4",

}

Visualization of vasculature using a hand-held photoacoustic probe: phantom and in vivo validation. / Heres, H.M.; Arabul, M.Ü.; Rutten, M.C.M.; van de Vosse, F.N.; Lopata, R.G.P.

In: Journal of Biomedical Optics, Vol. 22, No. 4, 041013, 04.2017, p. 1-8.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Visualization of vasculature using a hand-held photoacoustic probe: phantom and in vivo validation

AU - Heres, H.M.

AU - Arabul, M.Ü.

AU - Rutten, M.C.M.

AU - van de Vosse, F.N.

AU - Lopata, R.G.P.

PY - 2017/4

Y1 - 2017/4

N2 - Assessment of microvasculature and tissue perfusion can provide diagnostic information on local or systemic diseases. Photoacoustic (PA) imaging has strong clinical potential because of its sensitivity to hemoglobin. We used a hand-held PA probe with integrated diode lasers and examined its feasibility and validity in the detection of increasing blood volume and (sub) dermal vascularization. Blood volume detection was tested in custom-made perfusion phantoms. Results showed that an increase of blood volume in a physiological range of 1.3% to 5.4% could be detected. The results were validated with power Doppler sonography. Using a motorized scanning setup, areas of the skin were imaged at relatively short scanning times (<10  s/cm 2 <10  s/cm2 ) with PA. Three-dimensional visualization of these structures was achieved by combining the consecutively acquired cross-sectional images. Images revealed the epidermis and submillimeter vasculature up to depth of 5 mm. The geometries of imaged vasculature were validated with segmentation of the vasculature in high-frequency ultrasound imaging. This study proves the feasibility of PA imaging in its current implementation for the detection of perfusion-related parameters in skin and subdermal tissue and underlines its potential as a diagnostic tool in vascular or dermal pathologies.

AB - Assessment of microvasculature and tissue perfusion can provide diagnostic information on local or systemic diseases. Photoacoustic (PA) imaging has strong clinical potential because of its sensitivity to hemoglobin. We used a hand-held PA probe with integrated diode lasers and examined its feasibility and validity in the detection of increasing blood volume and (sub) dermal vascularization. Blood volume detection was tested in custom-made perfusion phantoms. Results showed that an increase of blood volume in a physiological range of 1.3% to 5.4% could be detected. The results were validated with power Doppler sonography. Using a motorized scanning setup, areas of the skin were imaged at relatively short scanning times (<10  s/cm 2 <10  s/cm2 ) with PA. Three-dimensional visualization of these structures was achieved by combining the consecutively acquired cross-sectional images. Images revealed the epidermis and submillimeter vasculature up to depth of 5 mm. The geometries of imaged vasculature were validated with segmentation of the vasculature in high-frequency ultrasound imaging. This study proves the feasibility of PA imaging in its current implementation for the detection of perfusion-related parameters in skin and subdermal tissue and underlines its potential as a diagnostic tool in vascular or dermal pathologies.

KW - photoacoustic imaging

KW - vasculature

KW - validation

KW - phantom

KW - probe

U2 - 10.1117/1.JBO.22.4.041013

DO - 10.1117/1.JBO.22.4.041013

M3 - Article

C2 - 28117865

VL - 22

SP - 1

EP - 8

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 4

M1 - 041013

ER -