Abstract
Ratiometric fluorescent sensor proteins with a very high and tunable affinity for Zn(II) were created by connecting two fluorescently labeled metal binding domains, CFP-Atox1 and WD4-YFP, using a series of flexible peptide linkers. A simple random-coil model describing the conformational distribution of the linker allowed a quant. understanding of the effect of the linker length on both the change in emission ratio and the Zn(II) affinity.
Original language | English |
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Pages (from-to) | 3494-3495 |
Journal | Journal of the American Chemical Society |
Volume | 129 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2007 |