Variation of linker length in ratiometric fluorescent sensor proteins allows rational tuning of Zn(II) affinity in the picomolar to femtomolar range

E.M.W.M. Dongen, van, T.H. Evers, L.M. Dekkers, E.W. Meijer, L.W.J. Klomp, M. Merkx

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Abstract

Ratiometric fluorescent sensor proteins with a very high and tunable affinity for Zn(II) were created by connecting two fluorescently labeled metal binding domains, CFP-Atox1 and WD4-YFP, using a series of flexible peptide linkers. A simple random-coil model describing the conformational distribution of the linker allowed a quant. understanding of the effect of the linker length on both the change in emission ratio and the Zn(II) affinity.
Original languageEnglish
Pages (from-to)3494-3495
JournalJournal of the American Chemical Society
Volume129
Issue number12
DOIs
Publication statusPublished - 2007

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