Personal cooling systems provide cooling for individual office occupants to maintain thermal comfort at their workplace when cooling is needed. The indoor temperature of the office can be maintained at several degrees higher than is customary in offices today when personal cooling is available, which results in energy saving for office buildings as a whole. To better understand the individual cooling demand of building occupants and develop good control strategies for personal cooling systems, it is necessary to assess the interaction between the user and the personal cooling system. For this purpose, a personal cooling system was tested in a stable, slightly warm environment (27.5°C) in a climate chamber with 11 human subjects. The personal cooling system was controlled by the subject using a simple slider. The interaction of the user with the system was related to comfort level and perceived air quality. The subjects are categorized into groups based on gender, on comfort level, and on whether their comfort improved during the test or not. The results show that comfort level did not directly reflect in a difference in the number of interactions or level of the setting. The largest difference in setting was found between male and female subjects, where females required less cooling.