UPLC-orbitrap-MS/MS based characterization of phytochemical compounds from Malaysia purple corn (Zea mays)

Ting Hun Lee; Chia Hau Lee; Syieluing Wong; Pei Ying Ong; Norfadilah Hamdan; Nurul Alia Azmi

doi:10.1016/j.bcab.2021.101922

UPLC-orbitrap-MS/MS based characterization of phytochemical compounds from Malaysia purple corn (Zea mays)

Ting Hun Lee (Corresponding author), Chia Hau Lee, Syieluing Wong, Pei Ying Ong, Norfadilah Hamdan, Nurul Alia Azmi

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

Purple corn (Zea mays) is one of the newly introduced important cash crops in Malaysia. It is a fast turnover crop, health-promoting that partially due to the secondary metabolite composition. However, little is known on its content and its secondary metabolite since it is first planted in Malaysia. Purple corn kernels were extracted in 1.5 N HCl-95% ethanol and the extracted compounds were separated on a C₁₈ column followed by an extensive characterization using UPLC-Orbitrap-MS/MS method in two ionization modes. A total of 12 metabolite compounds belong to different chemical groups (phenolic, flavonoid, alkaloid, ester, and fatty acid) and other organic compounds were tentatively identified, offering the first qualitative analysis available on the phytochemicals from purple corn. The data obtained in this study will provide a better understanding on the chemical composition of purple corn in Malaysia and hence, it could be used in food product developments and nutraceutical research based on this newly introduced crop.

Original language	English
Article number	101922
Number of pages	8
Journal	Biocatalysis and Agricultural Biotechnology
Volume	32
DOIs	https://doi.org/10.1016/j.bcab.2021.101922
Publication status	Published - Mar 2021
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank the Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM) for providing necessary facilities in order to complete experimental work. All the auhors are grateful to the UTM for supporting and providing financial assistance (Industry/International Incentive Grant) with vot: Q.J130000.3051.01M26.

Funding

The authors would like to thank the Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM) for providing necessary facilities in order to complete experimental work. All the auhors are grateful to the UTM for supporting and providing financial assistance (Industry/International Incentive Grant) with vot: Q.J130000.3051.01M26.

Keywords

Characterization
Malaysia
Phytochemical compound
Purple corn
UPLC-Orbitrap-MS/MS

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10.1016/j.bcab.2021.101922

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title = "UPLC-orbitrap-MS/MS based characterization of phytochemical compounds from Malaysia purple corn (Zea mays)",

abstract = "Purple corn (Zea mays) is one of the newly introduced important cash crops in Malaysia. It is a fast turnover crop, health-promoting that partially due to the secondary metabolite composition. However, little is known on its content and its secondary metabolite since it is first planted in Malaysia. Purple corn kernels were extracted in 1.5 N HCl-95% ethanol and the extracted compounds were separated on a C18 column followed by an extensive characterization using UPLC-Orbitrap-MS/MS method in two ionization modes. A total of 12 metabolite compounds belong to different chemical groups (phenolic, flavonoid, alkaloid, ester, and fatty acid) and other organic compounds were tentatively identified, offering the first qualitative analysis available on the phytochemicals from purple corn. The data obtained in this study will provide a better understanding on the chemical composition of purple corn in Malaysia and hence, it could be used in food product developments and nutraceutical research based on this newly introduced crop.",

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AU - Ong, Pei Ying

AU - Hamdan, Norfadilah

AU - Azmi, Nurul Alia

N1 - Funding Information: The authors would like to thank the Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM) for providing necessary facilities in order to complete experimental work. All the auhors are grateful to the UTM for supporting and providing financial assistance (Industry/International Incentive Grant) with vot: Q.J130000.3051.01M26.

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Y1 - 2021/3

N2 - Purple corn (Zea mays) is one of the newly introduced important cash crops in Malaysia. It is a fast turnover crop, health-promoting that partially due to the secondary metabolite composition. However, little is known on its content and its secondary metabolite since it is first planted in Malaysia. Purple corn kernels were extracted in 1.5 N HCl-95% ethanol and the extracted compounds were separated on a C18 column followed by an extensive characterization using UPLC-Orbitrap-MS/MS method in two ionization modes. A total of 12 metabolite compounds belong to different chemical groups (phenolic, flavonoid, alkaloid, ester, and fatty acid) and other organic compounds were tentatively identified, offering the first qualitative analysis available on the phytochemicals from purple corn. The data obtained in this study will provide a better understanding on the chemical composition of purple corn in Malaysia and hence, it could be used in food product developments and nutraceutical research based on this newly introduced crop.

AB - Purple corn (Zea mays) is one of the newly introduced important cash crops in Malaysia. It is a fast turnover crop, health-promoting that partially due to the secondary metabolite composition. However, little is known on its content and its secondary metabolite since it is first planted in Malaysia. Purple corn kernels were extracted in 1.5 N HCl-95% ethanol and the extracted compounds were separated on a C18 column followed by an extensive characterization using UPLC-Orbitrap-MS/MS method in two ionization modes. A total of 12 metabolite compounds belong to different chemical groups (phenolic, flavonoid, alkaloid, ester, and fatty acid) and other organic compounds were tentatively identified, offering the first qualitative analysis available on the phytochemicals from purple corn. The data obtained in this study will provide a better understanding on the chemical composition of purple corn in Malaysia and hence, it could be used in food product developments and nutraceutical research based on this newly introduced crop.

KW - Characterization

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KW - Phytochemical compound

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KW - UPLC-Orbitrap-MS/MS

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ER -

UPLC-orbitrap-MS/MS based characterization of phytochemical compounds from Malaysia purple corn (Zea mays)

Abstract

Bibliographical note

Funding

Keywords

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