Unmixing multi-spectral photoacoustic sources in human carotid plaques using non-negative independent component analysis

M.U. Arabul, M.C.M. Rutten, P. Bruneval, M.R.H.M. van Sambeek, F.N. van de Vosse, R.G.P. Lopata (Corresponding author)

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Abstract

Multi-spectral photoacoustic imaging (MSPAI) is promising for morphology assessment of carotid plaques; however, obtaining unique spectral characteristics of chromophores is cumbersome. We used MSPAI and non-negative independent component analysis (ICA) to unmix distinct signal sources in human carotid plaques blindly. The feasibility of the method was demonstrated on a plaque phantom with hemorrhage and cholesterol inclusions, and plaque endarterectomy samples ex vivo. Furthermore, the results were verified with histology using Masson's trichrome staining. Results showed that ICA could separate recent hemorrhages from old hemorrhages. Additionally, the signatures of cholesterol inclusion were also captured for the phantom experiment. Artifacts were successfully removed from signal sources. Histologic examinations showed high resemblance with the unmixed components and confirmed the morphologic distinction between recent and mature hemorrhages. In future pre-clinical studies, unmixing could be used for morphology assessment of intact human plaque samples.

Original languageEnglish
Article number100140
Number of pages8
JournalPhotoacoustics
Volume15
DOIs
Publication statusPublished - 1 Sep 2019

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hemorrhages
Hemorrhage
cholesterol
Cholesterol
inclusions
Endarterectomy
histology
staining
Artifacts
chromophores
artifacts
Histology
examination
signatures
Staining and Labeling

Keywords

  • Atherosclerosis
  • Blind unmixing
  • Histology
  • Independent component analysis
  • Morphology
  • Photoacoustic imaging
  • Vulnerable plaques

Cite this

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title = "Unmixing multi-spectral photoacoustic sources in human carotid plaques using non-negative independent component analysis",
abstract = "Multi-spectral photoacoustic imaging (MSPAI) is promising for morphology assessment of carotid plaques; however, obtaining unique spectral characteristics of chromophores is cumbersome. We used MSPAI and non-negative independent component analysis (ICA) to unmix distinct signal sources in human carotid plaques blindly. The feasibility of the method was demonstrated on a plaque phantom with hemorrhage and cholesterol inclusions, and plaque endarterectomy samples ex vivo. Furthermore, the results were verified with histology using Masson's trichrome staining. Results showed that ICA could separate recent hemorrhages from old hemorrhages. Additionally, the signatures of cholesterol inclusion were also captured for the phantom experiment. Artifacts were successfully removed from signal sources. Histologic examinations showed high resemblance with the unmixed components and confirmed the morphologic distinction between recent and mature hemorrhages. In future pre-clinical studies, unmixing could be used for morphology assessment of intact human plaque samples.",
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AU - Rutten, M.C.M.

AU - Bruneval, P.

AU - van Sambeek, M.R.H.M.

AU - van de Vosse, F.N.

AU - Lopata, R.G.P.

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N2 - Multi-spectral photoacoustic imaging (MSPAI) is promising for morphology assessment of carotid plaques; however, obtaining unique spectral characteristics of chromophores is cumbersome. We used MSPAI and non-negative independent component analysis (ICA) to unmix distinct signal sources in human carotid plaques blindly. The feasibility of the method was demonstrated on a plaque phantom with hemorrhage and cholesterol inclusions, and plaque endarterectomy samples ex vivo. Furthermore, the results were verified with histology using Masson's trichrome staining. Results showed that ICA could separate recent hemorrhages from old hemorrhages. Additionally, the signatures of cholesterol inclusion were also captured for the phantom experiment. Artifacts were successfully removed from signal sources. Histologic examinations showed high resemblance with the unmixed components and confirmed the morphologic distinction between recent and mature hemorrhages. In future pre-clinical studies, unmixing could be used for morphology assessment of intact human plaque samples.

AB - Multi-spectral photoacoustic imaging (MSPAI) is promising for morphology assessment of carotid plaques; however, obtaining unique spectral characteristics of chromophores is cumbersome. We used MSPAI and non-negative independent component analysis (ICA) to unmix distinct signal sources in human carotid plaques blindly. The feasibility of the method was demonstrated on a plaque phantom with hemorrhage and cholesterol inclusions, and plaque endarterectomy samples ex vivo. Furthermore, the results were verified with histology using Masson's trichrome staining. Results showed that ICA could separate recent hemorrhages from old hemorrhages. Additionally, the signatures of cholesterol inclusion were also captured for the phantom experiment. Artifacts were successfully removed from signal sources. Histologic examinations showed high resemblance with the unmixed components and confirmed the morphologic distinction between recent and mature hemorrhages. In future pre-clinical studies, unmixing could be used for morphology assessment of intact human plaque samples.

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