Threshold sensing through a synthetic enzymatic reaction-diffusion network

S. N. Semenov, A.J. Markvoort, T.F.A. Greef, de, W.T.S. Huck

Research output: Contribution to journalArticleAcademicpeer-review

37 Citations (Scopus)
6 Downloads (Pure)


A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks.
Original languageEnglish
Pages (from-to)8806-8069
Number of pages4
JournalAngewandte Chemie - International Edition
Issue number31
Publication statusPublished - 2014


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