The relative contributions of compression and hypoxia to development of muscle tissue damage: an in vitro study

D. Gawlitta, Wei Li, C.W.J. Oomens, F.P.T. Baaijens, D.L. Bader, C.V.C. Bouten

Research output: Contribution to journalArticleAcademicpeer-review

135 Citations (Scopus)
1 Downloads (Pure)

Abstract

Deep pressure ulcers develop in tissues subjected to sustained mechanical loading. Though it has been hypothesized that this damage mechanism results from local tissue ischemia, it has recently been shown with a cell model that sustained compression can cause cell deformation, leading to tissue breakdown. The present study focuses on the assessment of cell viability during compression and ischemia in an in vitro muscle model to determine their relative contributions to damage development.A model system was developed consisting of engineered skeletal muscle produced from the culture of murine muscle cells in a collagen gel. The tissue was subjected to 0, 20, or 40% compression under hypoxic or normoxic conditions. Experiments were performed on the stage of a microscope and cell viability was monitored using fluorescent markers for apoptotic andnecrotic cell death.Hypoxia did not lead to significant cell death over a 22 hour period. By contrast, compression led to immediate cell death that increased with time. No additional effect of hypoxia on cell death wasobserved. These data show that contrary to existing theories, compression can cause development of muscle damage and that hypoxia does not contribute to cell death development within 22 hours in engineered muscle.
Original languageEnglish
Pages (from-to)273-284
JournalAnnals of Biomedical Engineering
Volume35
Issue number2
DOIs
Publication statusPublished - 2007

Fingerprint Dive into the research topics of 'The relative contributions of compression and hypoxia to development of muscle tissue damage: an in vitro study'. Together they form a unique fingerprint.

Cite this