TY - JOUR
T1 - The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds
AU - Gerhardt, L.C.
AU - Widdows, K.L
AU - Erol, M.M.
AU - Burch, C.W.
AU - Sanz-Herrera, J.A.
AU - Ochoa, I.
AU - Staempfli, R.
AU - Roqan, I.S.
AU - Gabe, S.
AU - Ansari, T.
AU - Boccaccini, A.R.
PY - 2011
Y1 - 2011
N2 - The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81 – 93 %), permeability (k = 5.4 – 8.6 × 10-9 m2) and compressive strength values (0.4 – 1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6 – 15.1 %) than PDLLA scaffolds (8.5 %). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct.
AB - The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81 – 93 %), permeability (k = 5.4 – 8.6 × 10-9 m2) and compressive strength values (0.4 – 1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6 – 15.1 %) than PDLLA scaffolds (8.5 %). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct.
U2 - 10.1016/j.biomaterials.2011.02.032
DO - 10.1016/j.biomaterials.2011.02.032
M3 - Article
C2 - 21411138
SN - 0142-9612
VL - 32
SP - 4096
EP - 4108
JO - Biomaterials
JF - Biomaterials
IS - 17
ER -