The melanopic sensitivity function accounts for melanopsin-driven responses in mice under diverse lighting conditions

Timothy M. Brown, Annette E. Allen, Jazi Al-Enezi, Jonathan Wynne, Luc Schlangen, Vanja Hommes, Robert J. Lucas

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)
5 Downloads (Pure)

Abstract

In addition to rods and cones, photoreception in mammals extends to a third retinal cell type expressing the photopigment melanopsin. The influences of this novel opsin are widespread, ranging from pupillary and circadian responses to brightness perception, yet established approaches to quantifying the biological effects of light do not adequately account for melanopsin sensitivity. We have recently proposed a novel metric, the melanopic sensitivity function (VZλ), to address this deficiency. Here, we further validate this new measure with a variety of tests based on potential barriers to its applicability identified in the literature or relating to obvious practical benefits. Using electrophysiogical approaches and pupillometry, initially in rodless+coneless mice, our data demonstrate that under a very wide range of different conditions (including switching between stimuli with highly divergent spectral content) the VZλ function provides an accurate prediction of the sensitivity of melanopsin-dependent responses. We further show that VZλ provides the best available description of the spectral sensitivity of at least one aspect of the visual response in mice with functional rods and cones: tonic firing activity in the lateral geniculate nuclei. Together, these data establish VZλ as an important new approach for light measurement with widespread practical utility.

Original languageEnglish
Article numberA2400
JournalPLoS ONE
Volume8
Issue number1
DOIs
Publication statusPublished - 3 Jan 2013
Externally publishedYes

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