The initial stages of template-controlled CaCO3 formation revealed by Cryo-TEM

E.M. Pouget, P.H.H. Bomans, J.A.C.M. Goos, P.M. Frederik, G. With, de, N.A.J.M. Sommerdijk

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Abstract

Biogenic calcium carbonate forms the inorganic component of seashells, otoliths, and many marineskeletons, and its formation is directed by an ordered template of macromolecules. Classicalnucleation theory considers crystal formation to occur from a critical nucleus formed by theassembly of ions from solution. Using cryotransmission electron microscopy, we found thattemplate-directed calcium carbonate formation starts with the formation of prenucleation clusters.Their aggregation leads to the nucleation of amorphous nanoparticles in solution. Thesenanoparticles assemble at the template and, after reaching a critical size, develop dynamiccrystalline domains, one of which is selectively stabilized by the template. Our findings haveimplications for template-directed mineral formation in biological as well as in synthetic systems.
Original languageEnglish
Pages (from-to)1455-1458
JournalScience
Volume323
Issue number5920
DOIs
Publication statusPublished - 2009

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calcium carbonate
transmission electron microscopy
otolith
electron microscopy
nucleation
crystal
ion
mineral
nanoparticle

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Pouget, E. M., Bomans, P. H. H., Goos, J. A. C. M., Frederik, P. M., With, de, G., & Sommerdijk, N. A. J. M. (2009). The initial stages of template-controlled CaCO3 formation revealed by Cryo-TEM. Science, 323(5920), 1455-1458. https://doi.org/10.1126/science.1169434
Pouget, E.M. ; Bomans, P.H.H. ; Goos, J.A.C.M. ; Frederik, P.M. ; With, de, G. ; Sommerdijk, N.A.J.M. / The initial stages of template-controlled CaCO3 formation revealed by Cryo-TEM. In: Science. 2009 ; Vol. 323, No. 5920. pp. 1455-1458.
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abstract = "Biogenic calcium carbonate forms the inorganic component of seashells, otoliths, and many marineskeletons, and its formation is directed by an ordered template of macromolecules. Classicalnucleation theory considers crystal formation to occur from a critical nucleus formed by theassembly of ions from solution. Using cryotransmission electron microscopy, we found thattemplate-directed calcium carbonate formation starts with the formation of prenucleation clusters.Their aggregation leads to the nucleation of amorphous nanoparticles in solution. Thesenanoparticles assemble at the template and, after reaching a critical size, develop dynamiccrystalline domains, one of which is selectively stabilized by the template. Our findings haveimplications for template-directed mineral formation in biological as well as in synthetic systems.",
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Pouget, EM, Bomans, PHH, Goos, JACM, Frederik, PM, With, de, G & Sommerdijk, NAJM 2009, 'The initial stages of template-controlled CaCO3 formation revealed by Cryo-TEM', Science, vol. 323, no. 5920, pp. 1455-1458. https://doi.org/10.1126/science.1169434

The initial stages of template-controlled CaCO3 formation revealed by Cryo-TEM. / Pouget, E.M.; Bomans, P.H.H.; Goos, J.A.C.M.; Frederik, P.M.; With, de, G.; Sommerdijk, N.A.J.M.

In: Science, Vol. 323, No. 5920, 2009, p. 1455-1458.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Pouget, E.M.

AU - Bomans, P.H.H.

AU - Goos, J.A.C.M.

AU - Frederik, P.M.

AU - With, de, G.

AU - Sommerdijk, N.A.J.M.

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AB - Biogenic calcium carbonate forms the inorganic component of seashells, otoliths, and many marineskeletons, and its formation is directed by an ordered template of macromolecules. Classicalnucleation theory considers crystal formation to occur from a critical nucleus formed by theassembly of ions from solution. Using cryotransmission electron microscopy, we found thattemplate-directed calcium carbonate formation starts with the formation of prenucleation clusters.Their aggregation leads to the nucleation of amorphous nanoparticles in solution. Thesenanoparticles assemble at the template and, after reaching a critical size, develop dynamiccrystalline domains, one of which is selectively stabilized by the template. Our findings haveimplications for template-directed mineral formation in biological as well as in synthetic systems.

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