The influence of the cytosolic oncotic pressure on the permeability of the mitochondrial outer membrane for ADP

F.N. Gellerich, M. Kapischke, W. Kunz, W. Neumann, A. Kuznetsov, D. Brdiczka, K. Nicolaij

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Abstract

Cytosolic proteins as components of the physiological mitochondrial environment were substituted by dextrans added to media normally used for incubation of isolated mitochondria. Under these conditions the volume of the intermembrane space decreases and the contact sites between the both mitochondrial membranes increase drastically. These morphological changes are accompanied by a reduced permeability of the mitochondrial outer compartment for adenine nucleotides as it was shown by extensive kinetic studies of mitochondrial enzymes (oxidative phosphorylation, mi-creatine kinase, mi-adenylate kinase). The decreased permeability of the mitochondrial outer membrane causes increased rate dependent concentration gradients in the micromolar range for adenine nucleotides between the intermembrane space and the extramitochondrial space. Although all metabolites crossing the outer membrane exhibit the same concentration gradients, considerable compartmentations are detectable for ADP only due to its low extramitochondrial concentration. The consequences of ADP-compartmentation in the mitochondrial intermembrane space for ADP-channelling into the mitochondria are discussed.
Original languageEnglish
Pages (from-to)85-104
Number of pages20
JournalMolecular and Cellular Biochemistry
Volume133/1
DOIs
Publication statusPublished - 1994

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Mitochondrial Membranes
Adenosine Diphosphate
Permeability
Mitochondria
Adenine Nucleotides
Membranes
Pressure
Adenylate Kinase
Oxidative Phosphorylation
Creatine Kinase
Metabolites
Dextrans
Kinetics
Enzymes
Proteins

Cite this

Gellerich, F. N., Kapischke, M., Kunz, W., Neumann, W., Kuznetsov, A., Brdiczka, D., & Nicolaij, K. (1994). The influence of the cytosolic oncotic pressure on the permeability of the mitochondrial outer membrane for ADP. Molecular and Cellular Biochemistry, 133/1, 85-104. https://doi.org/10.1007/BF01267949
Gellerich, F.N. ; Kapischke, M. ; Kunz, W. ; Neumann, W. ; Kuznetsov, A. ; Brdiczka, D. ; Nicolaij, K. / The influence of the cytosolic oncotic pressure on the permeability of the mitochondrial outer membrane for ADP. In: Molecular and Cellular Biochemistry. 1994 ; Vol. 133/1. pp. 85-104.
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abstract = "Cytosolic proteins as components of the physiological mitochondrial environment were substituted by dextrans added to media normally used for incubation of isolated mitochondria. Under these conditions the volume of the intermembrane space decreases and the contact sites between the both mitochondrial membranes increase drastically. These morphological changes are accompanied by a reduced permeability of the mitochondrial outer compartment for adenine nucleotides as it was shown by extensive kinetic studies of mitochondrial enzymes (oxidative phosphorylation, mi-creatine kinase, mi-adenylate kinase). The decreased permeability of the mitochondrial outer membrane causes increased rate dependent concentration gradients in the micromolar range for adenine nucleotides between the intermembrane space and the extramitochondrial space. Although all metabolites crossing the outer membrane exhibit the same concentration gradients, considerable compartmentations are detectable for ADP only due to its low extramitochondrial concentration. The consequences of ADP-compartmentation in the mitochondrial intermembrane space for ADP-channelling into the mitochondria are discussed.",
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Gellerich, FN, Kapischke, M, Kunz, W, Neumann, W, Kuznetsov, A, Brdiczka, D & Nicolaij, K 1994, 'The influence of the cytosolic oncotic pressure on the permeability of the mitochondrial outer membrane for ADP', Molecular and Cellular Biochemistry, vol. 133/1, pp. 85-104. https://doi.org/10.1007/BF01267949

The influence of the cytosolic oncotic pressure on the permeability of the mitochondrial outer membrane for ADP. / Gellerich, F.N.; Kapischke, M.; Kunz, W.; Neumann, W.; Kuznetsov, A.; Brdiczka, D.; Nicolaij, K.

In: Molecular and Cellular Biochemistry, Vol. 133/1, 1994, p. 85-104.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - The influence of the cytosolic oncotic pressure on the permeability of the mitochondrial outer membrane for ADP

AU - Gellerich, F.N.

AU - Kapischke, M.

AU - Kunz, W.

AU - Neumann, W.

AU - Kuznetsov, A.

AU - Brdiczka, D.

AU - Nicolaij, K.

PY - 1994

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AB - Cytosolic proteins as components of the physiological mitochondrial environment were substituted by dextrans added to media normally used for incubation of isolated mitochondria. Under these conditions the volume of the intermembrane space decreases and the contact sites between the both mitochondrial membranes increase drastically. These morphological changes are accompanied by a reduced permeability of the mitochondrial outer compartment for adenine nucleotides as it was shown by extensive kinetic studies of mitochondrial enzymes (oxidative phosphorylation, mi-creatine kinase, mi-adenylate kinase). The decreased permeability of the mitochondrial outer membrane causes increased rate dependent concentration gradients in the micromolar range for adenine nucleotides between the intermembrane space and the extramitochondrial space. Although all metabolites crossing the outer membrane exhibit the same concentration gradients, considerable compartmentations are detectable for ADP only due to its low extramitochondrial concentration. The consequences of ADP-compartmentation in the mitochondrial intermembrane space for ADP-channelling into the mitochondria are discussed.

U2 - 10.1007/BF01267949

DO - 10.1007/BF01267949

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