TY - JOUR
T1 - The epicardium as modulator of the cardiac autonomic response during early development
AU - Kelder, Tim P.
AU - Duim, Sjoerd N.
AU - Vicente-Steijn, Rebecca
AU - Végh, Anna M.D.
AU - Kruithof, Boudewijn P.T.
AU - Smits, Anke M.
AU - van Bavel, Thomas C.
AU - Bax, Noortje
AU - Schalij, Martin J.
AU - Gittenberger-de Groot, Adriana C.
AU - DeRuiter, Marco C.
AU - Goumans, M.J.T.H
AU - Jongbloed, Monique R.M.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The cardiac autonomic nervous system (cANS) modulates heart rate, contraction force and conduction velocity. The embryonic chicken heart already responds to epinephrine prior to establishment of the cANS. The aim of this study was to define the regions of the heart that might participate in modulating the early autonomic response to epinephrine. Immunofluorescence analysis reveals expression of neural markers tubulin beta-3 chain and neural cell adhesion molecule in the epicardium during early development. In addition, expression of the β2 adrenergic receptor, the receptor for epinephrine, was found in the epicardium. Ex-ovo micro-electrode recordings in hearts with inhibition of epicardial outgrowth showed a significantly reduced response of the heart rate to epinephrine compared to control hearts. This study suggests a role for the epicardium as autonomic modulator during early cardiac development.
AB - The cardiac autonomic nervous system (cANS) modulates heart rate, contraction force and conduction velocity. The embryonic chicken heart already responds to epinephrine prior to establishment of the cANS. The aim of this study was to define the regions of the heart that might participate in modulating the early autonomic response to epinephrine. Immunofluorescence analysis reveals expression of neural markers tubulin beta-3 chain and neural cell adhesion molecule in the epicardium during early development. In addition, expression of the β2 adrenergic receptor, the receptor for epinephrine, was found in the epicardium. Ex-ovo micro-electrode recordings in hearts with inhibition of epicardial outgrowth showed a significantly reduced response of the heart rate to epinephrine compared to control hearts. This study suggests a role for the epicardium as autonomic modulator during early cardiac development.
KW - Autonomic modulation
KW - Autonomic nervous system
KW - Cardiac conduction system
KW - Cardiac development
KW - Epicardium
UR - http://www.scopus.com/inward/record.url?scp=84952652335&partnerID=8YFLogxK
U2 - 10.1016/j.yjmcc.2015.10.025
DO - 10.1016/j.yjmcc.2015.10.025
M3 - Article
C2 - 26527381
AN - SCOPUS:84952652335
SN - 0022-2828
VL - 89
SP - 251
EP - 259
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
ER -