The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.
|Title of host publication||Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985|
|Editors||P. Sandra, W. Bertsch|
|Place of Publication||Heidelberg|
|Publication status||Published - 1985|
Bruijn, de, E. A., Leclercq, P. A., & Tjaden, U. R. (1985). The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography. In P. Sandra, & W. Bertsch (Eds.), Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985 (pp. 599-608). Huethig.