Abstract
The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied.
Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985 |
| Editors | P. Sandra, W. Bertsch |
| Place of Publication | Heidelberg |
| Publisher | Huethig |
| Pages | 599-608 |
| Publication status | Published - 1985 |
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Bruijn, de, E. A., Leclercq, P. A., & Tjaden, U. R. (1985). The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography. In P. Sandra, & W. Bertsch (Eds.), Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985 (pp. 599-608). Huethig.