The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography

E.A. Bruijn, de, P.A. Leclercq, U.R. Tjaden

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Abstract

The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.
Original languageEnglish
Title of host publicationProceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985
EditorsP. Sandra, W. Bertsch
Place of PublicationHeidelberg
PublisherHuethig
Pages599-608
Publication statusPublished - 1985

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    Bruijn, de, E. A., Leclercq, P. A., & Tjaden, U. R. (1985). The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography. In P. Sandra, & W. Bertsch (Eds.), Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985 (pp. 599-608). Huethig.