The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography

E.A. Bruijn, de; P.A. Leclercq; U.R. Tjaden

The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography

E.A. Bruijn, de, P.A. Leclercq, U.R. Tjaden

Chemical Engineering and Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.

Original language	English
Title of host publication	Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985
Editors	P. Sandra, W. Bertsch
Place of Publication	Heidelberg
Publisher	Huethig
Pages	599-608
Publication status	Published - 1985

Cite this

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title = "The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography",

abstract = "The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.",

author = "{Bruijn, de}, E.A. and P.A. Leclercq and U.R. Tjaden",

year = "1985",

language = "English",

pages = "599--608",

editor = "P. Sandra and W. Bertsch",

booktitle = "Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985",

publisher = "Huethig",

}

The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography. / Bruijn, de, E.A.; Leclercq, P.A.; Tjaden, U.R.

Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985. ed. / P. Sandra; W. Bertsch. Heidelberg : Huethig, 1985. p. 599-608.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography

AU - Bruijn, de, E.A.

AU - Leclercq, P.A.

AU - Tjaden, U.R.

PY - 1985

Y1 - 1985

N2 - The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.

AB - The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.

M3 - Conference contribution

SP - 599

EP - 608

BT - Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985

A2 - Sandra, P.

A2 - Bertsch, W.

PB - Huethig

CY - Heidelberg

ER -

The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography

Abstract

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