Abstract
The selectivity of a capillary gas chromatographic assay for the anti-neoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-ketocyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies showed that a cyclization product of 4-keto-CPA can form using the same chromatographic conditions as those under which CPA was determined. However, the amount produced is relatively small (less than 10%) compared with the percentage produced from CPA. Furthermore, both cyclization products, formed by loss of HCI and intraalkylation of the parent compounds, can be separated well under suitable chromatographic conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 89-94 |
| Journal | HRC & CC, Journal of High Resolution Chromatography and Chromatography Communications |
| Volume | 9 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1986 |
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