TFMLAB: A MATLAB toolbox for 4D traction force microscopy

Jorge Barrasa-Fano; Apeksha Shapeti; Álvaro Jorge-Peñas; Mojtaba Barzegari; José Antonio Sanz-Herrera; Hans Van Oosterwyck

doi:10.1016/j.softx.2021.100723

TFMLAB: A MATLAB toolbox for 4D traction force microscopy

Jorge Barrasa-Fano, Apeksha Shapeti, Álvaro Jorge-Peñas, Mojtaba Barzegari, José Antonio Sanz-Herrera, Hans Van Oosterwyck (Corresponding author)

Research output: Contribution to journal › Article › Academic › peer-review

29 Citations (Scopus)

Abstract

We present TFMLAB, a MATLAB software package for 4D (x;y;z;t) Traction Force Microscopy (TFM). While various TFM computational workflows are available in the literature, open-source programs that are easy to use by researchers with limited technical experience and that can analyze 4D in vitro systems do not exist. TFMLAB integrates all the computational steps to compute active cellular forces from confocal microscopy images, including image processing, cell segmentation, image alignment, matrix displacement measurement and force recovery. Moreover, TFMLAB eases usability by means of interactive graphical user interfaces. This work describes the package's functionalities and analyzes its performance on a real TFM case.

Original language	English
Article number	100723
Number of pages	9
Journal	SoftwareX
Volume	15
DOIs	https://doi.org/10.1016/j.softx.2021.100723
Publication status	Published - Jul 2021
Externally published	Yes

Keywords

Cell mechanics
Finite element method
Image processing
Traction force microscopy

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10.1016/j.softx.2021.100723

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title = "TFMLAB: A MATLAB toolbox for 4D traction force microscopy",

abstract = "We present TFMLAB, a MATLAB software package for 4D (x;y;z;t) Traction Force Microscopy (TFM). While various TFM computational workflows are available in the literature, open-source programs that are easy to use by researchers with limited technical experience and that can analyze 4D in vitro systems do not exist. TFMLAB integrates all the computational steps to compute active cellular forces from confocal microscopy images, including image processing, cell segmentation, image alignment, matrix displacement measurement and force recovery. Moreover, TFMLAB eases usability by means of interactive graphical user interfaces. This work describes the package's functionalities and analyzes its performance on a real TFM case.",

keywords = "Cell mechanics, Finite element method, Image processing, Traction force microscopy",

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AU - Barrasa-Fano, Jorge

AU - Shapeti, Apeksha

AU - Jorge-Peñas, Álvaro

AU - Barzegari, Mojtaba

AU - Sanz-Herrera, José Antonio

AU - Van Oosterwyck, Hans

PY - 2021/7

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AB - We present TFMLAB, a MATLAB software package for 4D (x;y;z;t) Traction Force Microscopy (TFM). While various TFM computational workflows are available in the literature, open-source programs that are easy to use by researchers with limited technical experience and that can analyze 4D in vitro systems do not exist. TFMLAB integrates all the computational steps to compute active cellular forces from confocal microscopy images, including image processing, cell segmentation, image alignment, matrix displacement measurement and force recovery. Moreover, TFMLAB eases usability by means of interactive graphical user interfaces. This work describes the package's functionalities and analyzes its performance on a real TFM case.

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KW - Finite element method

KW - Image processing

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TFMLAB: A MATLAB toolbox for 4D traction force microscopy

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Keywords

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