Abstract
Multimodal imaging agents combine two or more imaging modalities into one probe. Self-assembling fluorescent nanoparticles are a promising class of modular multimodal imaging probes as they can allow easy blending of imaging and targeting modalities. Our group recently developed a class of self-assembling and intrinsically fluorescent small molecule-based nanoparticles (SMNPs) with excellent optical properties. In this article, we describe the efficient radiolabeling of these SMNPs via a two-step bioconjugation strategy involving the inverse-electron-demand Diels-Alder ligation between a tetrazine (Tz)-tagged radiolabel and a trans-cyclooctene (TCO)-tagged fluorescent small molecule building block of the SMNPs. Studies in mice revealed that the SMNPs are well tolerated and could be monitored by both radioactivity and fluorescence, thereby demonstrating the potential of SMNPs in optical and dual-mode imaging in vivo. The work also testifies to the utility of the Tz-TCO conjugation chemistry for the labeling of self-assembled nanoparticles.
| Original language | English |
|---|---|
| Pages (from-to) | 547–551 |
| Number of pages | 5 |
| Journal | Bioconjugate Chemistry |
| Volume | 30 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 20 Mar 2019 |
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Tetrazine - trans-cyclooctene chemistry applied to fabricate self-assembled fluorescent and radioactive nanoparticles for in vivo dual mode imaging. / van Onzen, Arthur H.A.M.; Rossin, Raffaella; Schenning, Albertus P.H.J.; Nicolay, Klaas; Milroy, Lech Gustav; Robillard, Marc S.; Brunsveld, Luc (Corresponding author).
In: Bioconjugate Chemistry, Vol. 30, No. 3, 20.03.2019, p. 547–551.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Tetrazine - trans-cyclooctene chemistry applied to fabricate self-assembled fluorescent and radioactive nanoparticles for in vivo dual mode imaging
AU - van Onzen, Arthur H.A.M.
AU - Rossin, Raffaella
AU - Schenning, Albertus P.H.J.
AU - Nicolay, Klaas
AU - Milroy, Lech Gustav
AU - Robillard, Marc S.
AU - Brunsveld, Luc
PY - 2019/3/20
Y1 - 2019/3/20
N2 - Multimodal imaging agents combine two or more imaging modalities into one probe. Self-assembling fluorescent nanoparticles are a promising class of modular multimodal imaging probes as they can allow easy blending of imaging and targeting modalities. Our group recently developed a class of self-assembling and intrinsically fluorescent small molecule-based nanoparticles (SMNPs) with excellent optical properties. In this article, we describe the efficient radiolabeling of these SMNPs via a two-step bioconjugation strategy involving the inverse-electron-demand Diels-Alder ligation between a tetrazine (Tz)-tagged radiolabel and a trans-cyclooctene (TCO)-tagged fluorescent small molecule building block of the SMNPs. Studies in mice revealed that the SMNPs are well tolerated and could be monitored by both radioactivity and fluorescence, thereby demonstrating the potential of SMNPs in optical and dual-mode imaging in vivo. The work also testifies to the utility of the Tz-TCO conjugation chemistry for the labeling of self-assembled nanoparticles.
AB - Multimodal imaging agents combine two or more imaging modalities into one probe. Self-assembling fluorescent nanoparticles are a promising class of modular multimodal imaging probes as they can allow easy blending of imaging and targeting modalities. Our group recently developed a class of self-assembling and intrinsically fluorescent small molecule-based nanoparticles (SMNPs) with excellent optical properties. In this article, we describe the efficient radiolabeling of these SMNPs via a two-step bioconjugation strategy involving the inverse-electron-demand Diels-Alder ligation between a tetrazine (Tz)-tagged radiolabel and a trans-cyclooctene (TCO)-tagged fluorescent small molecule building block of the SMNPs. Studies in mice revealed that the SMNPs are well tolerated and could be monitored by both radioactivity and fluorescence, thereby demonstrating the potential of SMNPs in optical and dual-mode imaging in vivo. The work also testifies to the utility of the Tz-TCO conjugation chemistry for the labeling of self-assembled nanoparticles.
UR - http://www.scopus.com/inward/record.url?scp=85062069610&partnerID=8YFLogxK
U2 - 10.1021/acs.bioconjchem.9b00038
DO - 10.1021/acs.bioconjchem.9b00038
M3 - Article
C2 - 30731039
AN - SCOPUS:85062069610
VL - 30
SP - 547
EP - 551
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
SN - 1043-1802
IS - 3
ER -