Terpolymer-stabilized complex coacervates: A robust and versatile synthetic cell platform

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

The utilization of liquid-liquid phase separated systems has seen increased attention as synthetic cell platforms due to their innate ability to sequester interesting, functional, and biologically relevant materials. However, their applications are limited by the temporal stability of such condensed phases. While there are a number of strategies toward droplet stabilization, in our group we have developed a polymer-based approach to stabilize complex coacervate microdroplets. These protocells are remarkably robust and have been utilized to support a number of new protocellular applications. Here, we describe in detail the methodologies we have developed for the synthesis of the starting components, their formation into stable, cargo-loaded protocells, and how these protocells are treated post-formation to purify and analyze the resultant functional self-assembled systems.

Original languageEnglish
Title of host publicationLiquid-Liquid Phase Coexistence and Membraneless Organelles
EditorsChristine D. Keating
PublisherAcademic Press Inc.
Chapter3
Pages51-82
Number of pages32
ISBN (Print)9780128211595
DOIs
Publication statusPublished - Jan 2021

Publication series

NameMethods in Enzymology
Volume646
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Coacervates
  • Compartmentalization
  • Polymer assembly
  • Protocells

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