Tendon-Derived Biomimetic Surface Topographies Induce Phenotypic Maintenance of Tenocytes in Vitro

Aysegul Dede Eren, Aliaksey Vasilevich, E Deniz Eren, Phanikrishna Sudarsanam, Urandelger Tuvshindorj, Jan de Boer, Jasper Foolen (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The tenocyte niche contains biochemical and biophysical signals that are needed for tendon homeostasis. The tenocyte phenotype is correlated with cell shape in vivo and in vitro, and shape-modifying cues are needed for tenocyte phenotypical maintenance. Indeed, cell shape changes from elongated to spread when cultured on a flat surface, and rat tenocytes lose the expression of phenotypical markers throughout five passages. We hypothesized that tendon gene expression can be preserved by culturing cells in the native tendon shape. To this end, we reproduced the tendon topographical landscape into tissue culture polystyrene, using imprinting technology. We confirmed that the imprints forced the cells into a more elongated shape, which correlated with the level of Scleraxis expression. When we cultured the tenocytes for 7 days on flat surfaces and tendon imprints, we observed a decline in tenogenic marker expression on flat but not on imprints. This research demonstrates that native tendon topography is an important factor contributing to the tenocyte phenotype. Tendon imprints therefore provide a powerful platform to explore the effect of instructive cues originating from native tendon topography on guiding cell shape, phenotype, and function of tendon-related cells.

Original languageEnglish
Pages (from-to)1023-1036
Number of pages14
JournalTissue engineering. Part A
Volume27
Issue number15-16
Early online date13 Nov 2020
DOIs
Publication statusPublished - 1 Aug 2021

Bibliographical note

Publisher Copyright:
© Copyright 2021, Mary Ann Liebert, Inc., publishers 2021.

Funding

This research has received funding from the European Union’s Horizon 2020 Research and Innovation Program under the Marie Sklodowska-Curie grant agreement No 676338.

FundersFunder number
Horizon 2020 Framework Programme
Marie Skłodowska‐Curie676338

    Keywords

    • dedifferentiation
    • redifferentiation
    • soft embossing
    • surface topography
    • tendon
    • tenocytes

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