Temperature-induced collapse of elastin-like peptides studied by 2DIR spectroscopy

Oleg Selig, Ana Maria de Carvalho Vicente da Cunha, Mark B. van Eldijk, Jan C.M. van Hest, Thomas L.C. Jansen, Huib J. Bakker, Yves Laurent Ariel Rezus

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)
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Abstract

Elastin-like peptides are hydrophobic biopolymers that exhibit a reversible coacervation transition when the temperature is raised above a critical point. Here, we use a combination of linear infrared spectroscopy, two-dimensional infrared spectroscopy, and molecular dynamics simulations to study the structural dynamics of two elastin-like peptides. Specifically, we investigate the effect of the solvent environment and temperature on the structural dynamics of a short (5-residue) elastin-like peptide and of a long (450-residue) elastin-like peptide. We identify two vibrational energy transfer processes that take place within the amide I′ band of both peptides. We observe that the rate constant of one of the exchange processes is strongly dependent on the solvent environment and argue that the coacervation transition is accompanied by a desolvation of the peptide backbone where up to 75% of the water molecules are displaced. We also study the spectral diffusion dynamics of the valine(1) residue that is present in both peptides. We find that these dynamics are relatively slow and indicative of an amide group that is shielded from the solvent. We conclude that the coacervation transition of elastin-like peptides is probably not associated with a conformational change involving this residue.

Original languageEnglish
Pages (from-to)8243-8254
Number of pages12
JournalJournal of Physical Chemistry B
Volume122
Issue number34
DOIs
Publication statusPublished - 30 Aug 2018

Keywords

  • Amides/chemistry
  • Hydrophobic and Hydrophilic Interactions
  • Molecular Dynamics Simulation
  • Peptides/chemistry
  • Phase Transition
  • Protein Conformation
  • Spectrophotometry, Infrared
  • Temperature
  • Vibration
  • Water/chemistry

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