Temperature-controlled positioning of fusion proteins in microreactors

R.L.M. Teeuwen; H. Zuilhof; F.A. de Wolf; J.C.M. van Hest

doi:10.1039/b903087e

Temperature-controlled positioning of fusion proteins in microreactors

R.L.M. Teeuwen
, H. Zuilhof
, F.A. de Wolf
, J.C.M. van Hest

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

We present a non-covalent immobilization system based on stimulus-responsive elastin-like polypeptides (ELPs) to facilitate the positioning of proteins in microchannels. Two ELP variants were constructed and connected to fluorescent proteins EGFP and DsRed2. These fusion proteins display an inverse transition behavior that can be simply controlled by varying concentrations of NaCl. With these ELP fusion proteins two patches of fluorescent proteins can be formed inside a microreactor, using only the temperature-responsive property of ELPs.

Original language	English
Pages (from-to)	2261-2268
Number of pages	8
Journal	Soft Matter
Volume	5
Issue number	11
DOIs	https://doi.org/10.1039/b903087e
Publication status	Published - 2009
Externally published	Yes

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abstract = "We present a non-covalent immobilization system based on stimulus-responsive elastin-like polypeptides (ELPs) to facilitate the positioning of proteins in microchannels. Two ELP variants were constructed and connected to fluorescent proteins EGFP and DsRed2. These fusion proteins display an inverse transition behavior that can be simply controlled by varying concentrations of NaCl. With these ELP fusion proteins two patches of fluorescent proteins can be formed inside a microreactor, using only the temperature-responsive property of ELPs.",

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AU - Teeuwen, R.L.M.

AU - Zuilhof, H.

AU - de Wolf, F.A.

AU - van Hest, J.C.M.

PY - 2009

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AB - We present a non-covalent immobilization system based on stimulus-responsive elastin-like polypeptides (ELPs) to facilitate the positioning of proteins in microchannels. Two ELP variants were constructed and connected to fluorescent proteins EGFP and DsRed2. These fusion proteins display an inverse transition behavior that can be simply controlled by varying concentrations of NaCl. With these ELP fusion proteins two patches of fluorescent proteins can be formed inside a microreactor, using only the temperature-responsive property of ELPs.

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DO - 10.1039/b903087e

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Temperature-controlled positioning of fusion proteins in microreactors

Abstract

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