Abstract
A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.
Original language | English |
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Pages (from-to) | 5197-5205 |
Journal | Nucleic Acids Research |
Volume | 18 |
Issue number | 17 |
DOIs | |
Publication status | Published - 1990 |