Synthesis of well-defined phosphate-methylated DNA fragments: the application of potassium carbonate in methanol as deprotecting reagent

W.H.A. Kuijpers, J. Huskens, L.H. Koole, C.A.A. Boeckel, van

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Abstract

A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.
Original languageEnglish
Pages (from-to)5197-5205
JournalNucleic Acids Research
Volume18
Issue number17
DOIs
Publication statusPublished - 1990

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Methanol
Phosphates
DNA
Oligodeoxyribonucleotides
Glass
potassium carbonate
9-fluorenylmethoxycarbonyl

Cite this

Kuijpers, W.H.A. ; Huskens, J. ; Koole, L.H. ; Boeckel, van, C.A.A. / Synthesis of well-defined phosphate-methylated DNA fragments: the application of potassium carbonate in methanol as deprotecting reagent. In: Nucleic Acids Research. 1990 ; Vol. 18, No. 17. pp. 5197-5205.
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Synthesis of well-defined phosphate-methylated DNA fragments: the application of potassium carbonate in methanol as deprotecting reagent. / Kuijpers, W.H.A.; Huskens, J.; Koole, L.H.; Boeckel, van, C.A.A.

In: Nucleic Acids Research, Vol. 18, No. 17, 1990, p. 5197-5205.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Synthesis of well-defined phosphate-methylated DNA fragments: the application of potassium carbonate in methanol as deprotecting reagent

AU - Kuijpers, W.H.A.

AU - Huskens, J.

AU - Koole, L.H.

AU - Boeckel, van, C.A.A.

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N2 - A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.

AB - A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.

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DO - 10.1093/nar/18.17.5197

M3 - Article

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SP - 5197

EP - 5205

JO - Nucleic Acids Research

JF - Nucleic Acids Research

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