Synthesis of well-defined phosphate-methylated DNA fragments: the application of potassium carbonate in methanol as deprotecting reagent

W.H.A. Kuijpers; J. Huskens; L.H. Koole; C.A.A. Boeckel, van

doi:10.1093/nar/18.17.5197

Synthesis of well-defined phosphate-methylated DNA fragments: the application of potassium carbonate in methanol as deprotecting reagent

W.H.A. Kuijpers, J. Huskens, L.H. Koole, C.A.A. Boeckel, van

Chemical Biology

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Abstract

A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.

Original language	English
Pages (from-to)	5197-5205
Journal	Nucleic Acids Research
Volume	18
Issue number	17
DOIs	https://doi.org/10.1093/nar/18.17.5197
Publication status	Published - 1990

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abstract = "A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.",

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AU - Kuijpers, W.H.A.

AU - Huskens, J.

AU - Koole, L.H.

AU - Boeckel, van, C.A.A.

PY - 1990

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N2 - A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.

AB - A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.

U2 - 10.1093/nar/18.17.5197

DO - 10.1093/nar/18.17.5197

M3 - Article

VL - 18

SP - 5197

EP - 5205

JO - Nucleic Acids Research

JF - Nucleic Acids Research

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ER -