Synthesis of well-defined phosphate-methylated DNA fragments: the application of potassium carbonate in methanol as deprotecting reagent

W.H.A. Kuijpers, J. Huskens, L.H. Koole, C.A.A. Boeckel, van

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Abstract

A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.
Original languageEnglish
Pages (from-to)5197-5205
JournalNucleic Acids Research
Volume18
Issue number17
DOIs
Publication statusPublished - 1990

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