Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 5197-5205 |
| Journal | Nucleic Acids Research |
| Volume | 18 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 1990 |
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Synthesis of well-defined phosphate-methylated DNA fragments: the application of potassium carbonate in methanol as deprotecting reagent. / Kuijpers, W.H.A.; Huskens, J.; Koole, L.H.; Boeckel, van, C.A.A.
In: Nucleic Acids Research, Vol. 18, No. 17, 1990, p. 5197-5205.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Synthesis of well-defined phosphate-methylated DNA fragments: the application of potassium carbonate in methanol as deprotecting reagent
AU - Kuijpers, W.H.A.
AU - Huskens, J.
AU - Koole, L.H.
AU - Boeckel, van, C.A.A.
PY - 1990
Y1 - 1990
N2 - A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.
AB - A new deprotection procedure in the synthesis of phosphate-methylated oligodeoxynucleotides involves treatment of fully protected DNA fragments with methanolic K2CO3. This reagent, for example, deblocks 9-fluorenylmethoxycarbonyl-protected bases and cyanomethyl-protected phosphotriesters and effectively removes the DNA oligomer from the controlled-pore glass support. Thus, DNA fragments which are phosphate-methylated at defined positions may be synthesized both in soln. and on a solid support.
U2 - 10.1093/nar/18.17.5197
DO - 10.1093/nar/18.17.5197
M3 - Article
VL - 18
SP - 5197
EP - 5205
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 17
ER -