Synthesis of palmitoylated Ras-peptides and -proteins

L. Brunsveld, J. Kuhlmann, H. Waldmann

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)
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In this review, an overview is given and details are provided for the synthesis of lipidated Ras (rat-adeno-sarcoma)-peptides and -proteins. The progress made in the synthesis of the lipidated peptides from the Ras superfamily is discussed with special emphasis on the recently developed solid-phase synthesis methods, since these methods have turned out to be the preferred synthesis method for the majority of the required peptides. Solid-phase lipopeptide synthesis has given access to native and modified peptides on a scale that allows peptide-consuming studies like for ligation to proteins and concomitant X-ray crystal structure determination. The access to these peptides has also enabled biological questions concerning these peptides and proteins to be resolved. The review describes different solid-phase methods, which are individually suited for different types of lipopeptides, differing for example in lipidation pattern or amino acid side-chain functionality, and their ligation to proteins. Finally, an example is provided how these peptides can serve to resolve biological aspects of the Ras family GTPases.
Original languageEnglish
Pages (from-to)151-165
Number of pages15
JournalMethods : a companion to methods in enzymology
Issue number2
Publication statusPublished - 2006

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